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  4. Recombinant Drosophila melanogaster Protein suppressor of underreplication (SuUR), partial

Recombinant Drosophila melanogaster Protein suppressor of underreplication (SuUR), partial

  • 中文名稱：
    黑腹果蠅SuUR重組蛋白
  • 貨號：
    CSB-YP896146DLU
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅SuUR重組蛋白
  • 貨號：
    CSB-EP896146DLU
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅SuUR重組蛋白
  • 貨號：
    CSB-EP896146DLU-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅SuUR重組蛋白
  • 貨號：
    CSB-BP896146DLU
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅SuUR重組蛋白
  • 貨號：
    CSB-MP896146DLU
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    SuUR
  • Uniprot No.：
  • 別名：
    SuUR; Su(UR)ES; CG7869; Protein suppressor of underreplication
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Required for underreplication of DNA, which is found in many late replicating euchromatic regions of salivary gland polytene chromosomes. Functions by promoting the localization and retention of Rif1 to active DNA replication forks where Rif1 inhibits replication fork progression. Controls chromatin organization in polytene chromosomes.
  • 基因功能參考文獻(xiàn)：
    1. Recruitment of either HP1(GAL4DBD) or SUUR(GAL4DBD) resulted in delayed completion of replication PMID: 25398563
    2. SUUR protein binding correlates with gene regulation and specific chromatin types. PMID: 24292500
    3. Our findings support the idea that the effect of SUUR on replication elongation depends on the cell cycle stage and can be mediated through its physical interaction with replication fork. PMID: 23149855
    4. Mutation of two conserved amino acid residues in this region impairs binding of SUUR to polytene chromosomes and reduces the ability of the protein to cause DNA underreplication. PMID: 19596903
    5. microdissection and sequence analysis of its pericentric heterochromatin PMID: 12111334
    6. SuUR mutation leads to earlier completion of DNA replication PMID: 12644953
    7. Differential responses of polyploid and diploid cells to ectopic SuUR may reflect differences in the mechanisms underlying mitotic cell cycles and endocycles PMID: 14508681
    8. SuUR binds specificialy to boundary regions and regular distribution of boundary regions in chromosomes may represent the dispersion of sites designed for chromosomal silencing PMID: 14702350
    9. SUUR has at least two separate chromosome-binding regions that are able to recognize intercalary and pericentric heterochromatin specifically. PMID: 16134332
    10. The SuUR gene affects the distribution of HP1 and SU(VAR)3-9 on the nurse cell poytene chromosomes. PMID: 16607511
    11. Data on the SuUR gene, its expression, and effect on polytene chromosome structure and replication are summarized. PMID: 17025152
    12. these results suggest that SUUR is a ubiquitous marker of heterochromatin in different cell types PMID: 17606990
    13. describe how, in double mutants for Suppressor of variegation 3-9 and SuUR genes encoding two structural proteins of heterochromatin, new banded heterochromatic segments appear in all polytene chromosomes PMID: 17640911
    14. Reversible decondensation of heterochromatin regions of polytene chromosomes during ectopic expression of the SuUR gene. PMID: 19650328
    15. Suppressor of Underreplication gene does not directely affect the replication progression PMID: 19817130

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  • 亞細(xì)胞定位：
    Nucleus. Chromosome.
  • 組織特異性：
    Expressed throughout development. Weakly expressed. Expressed at higher level in embryos and adult females.
  • 數(shù)據(jù)庫鏈接：

    KEGG: dme:Dmel_CG7869

    STRING: 7227.FBpp0075933

    UniGene: Dm.7710