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  4. Recombinant Drosophila melanogaster Werner Syndrome-like exonuclease (WRNexo)

Recombinant Drosophila melanogaster Werner Syndrome-like exonuclease (WRNexo)

  • 中文名稱：
    黑腹果蠅WRNexo重組蛋白
  • 貨號：
    CSB-YP893266DLU
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅WRNexo重組蛋白
  • 貨號：
    CSB-EP893266DLU
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅WRNexo重組蛋白
  • 貨號：
    CSB-EP893266DLU-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅WRNexo重組蛋白
  • 貨號：
    CSB-BP893266DLU
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅WRNexo重組蛋白
  • 貨號：
    CSB-MP893266DLU
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    WRNexo
  • Uniprot No.：
  • 別名：
    WRNexo; CG7670; Werner Syndrome-like exonuclease; DmWRNexo; EC 3.1.11.-; 3'-5' exonuclease
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    full length protein
  • 表達區(qū)域：
    1-353
  • 氨基酸序列
    MEKYLTKMPI KSKANEVPKE EAGVKKETPK VARKATKKDT PKELKDKENA GDDNTPKQTK GRPGRPAAKR KNLDTPDVTE KLAMEEENPP KRRSSRLTRS TRSMAEDGSP SPEKEKPEKL PFIKYKGAIK YFTESQDIAA SADDVLQWVE KQKDEVVPMA FDMEWPFSFQ TGPGKSAVIQ ICVDEKCCYI YQLTNVKKLP AALVALINHP KVRLHGVNIK NDFRKLARDF PEVTAEPLIE KCVDLGLWCN EVCETGGRWS LERLTNFIAK KAMDKSKKVR MSKWHVIPLD ENQLMYAAID VYIGQVIYRE LERREKVKIK NEEEFKEKNG DAAFKAMKAL GETFLTKINE VTL
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Essential for the formation of DNA replication focal centers. Has an important role in maintaining genome stability. Has exonuclease activity on both single-stranded and duplex templates bearing overhangs, but not blunt ended duplex DNA, and cleaves in a 3'-5' direction.
  • 基因功能參考文獻：
    1. WRNexo is important for recovery following both endogenous and exogenous replication stress; the critical role of WRNexo during replication stress does not depend on exonuclease activity PMID: 24709634
    2. The Drosophila WRN ortholog demonstrates an important role for WRN exonuclease in maintaining genome stability. PMID: 18346216
    3. DmWRNexo is a 3'-5' exonuclease: phenotypic and biochemical characterization of mutants of the Drosophila orthologue of human WRN exonuclease. PMID: 18956248
  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    WRNexo family
  • 數(shù)據(jù)庫鏈接：

    KEGG: dme:Dmel_CG7670

    STRING: 7227.FBpp0291513

    UniGene: Dm.4528