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  4. Recombinant Escherichia coli Flagellin (fliC)

Recombinant Escherichia coli Flagellin (fliC)

In Stock
  • 中文名稱：
    大腸桿菌fliC重組蛋白
  • 貨號(hào)：
    CSB-EP300968ENVe1
  • 規(guī)格：
    ￥3624
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP300968ENVe1 could indicate that this peptide derived from E.coli-expressed Escherichia coli (strain K12) fliC.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP300968ENVe1 could indicate that this peptide derived from E.coli-expressed Escherichia coli (strain K12) fliC.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    fliC
  • Uniprot No.：
  • 別名：
    fliC; flaF; hag; b1923; JW1908Flagellin
  • 種屬：
    Escherichia coli (strain K12)
  • 蛋白長度：
    Full Length of Mature Protein
  • 來源：
    E.coli
  • 分子量：
    51.2 kDa
  • 表達(dá)區(qū)域：
    2-498aa
  • 氨基酸序列
    AQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDDAAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRVRELTVQATTGTNSESDLSSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKNGSMKIQVGANDNQTITIDLKQIDAKTLGLDGFSVKNNDTVTTSAPVTAFGATTTNNIKLTGITLSTEAATDTGGTNPASIEGVYTDNGNDYYAKITGGDNDGKYYAVTVANDGTVTMATGATANATVTDANTTKATTITSGGTPVQIDNTAGSATANLGAVSLVKLQDSKGNDTDTYALKDTNGNLYAADVNETTGAVSVKTITYTDSSGAASSPTAVKLGGDDGKTEVVDIDGKTYDSADLNGGNLQTGLTAGGEALTAVANGKTTDPLKALDDAIASVDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標(biāo)簽：
    Tag-Free
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    Tris-based buffer,50% glycerol
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    3-7 business days
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella.
  • 基因功能參考文獻(xiàn)：
    1. Studies indicate that toll-like receptor 5 (TLR5) specifically interacts with flagellin, and is useful as an analytical recognition element for low concentrations of flagellin. PMID: 24881544
    2. Flagellin subunit FliC function as adhesins to piglet epithelial cells invasion. PMID: 23746569
    3. Results showed a reproducible and clear cut classification with molecular polymorphism of gene rfb cluster and flagella gene fliC. PMID: 19656466
    4. Flagellin in E. coli Nissle 1917 is the major stimulatory factor of human beta-defensin. PMID: 17283097
    5. Relations between the diversity of the fliC gene conditioning flagellum protein in E. coli and the source of the strain origin are presented PMID: 17702465
    6. Flagellin expression by uropathogenic Escherichia coli coincides with ascension of the ureters and colonization of the kidney. PMID: 17925449
    7. This study demonstrated that infection of intestinal epithelial cells by A/E pathogens can trigger an array of proinflammatory responses from epithelial cells through both FliC-dependent and -independent pathways. PMID: 18227166
    8. Insertion of Cys-derived thiol residues in the E. coli FliC protein increased Au(III) sorption and reduction onto the surface of the flagellar filament and resulted in the production of stabilized Au(0) nanoparticles of approximately 20-50 nm diameter. PMID: 18819156
    9. Binding of a fliC(H7) mutant O157 strain to rectal epithelium was significantly reduced as was binding of the flagellated wild-type strain following incubation with H7-specific antibodies. PMID: 19016776
    10. Increased IL-8 levels mainly depended on flagellin expression as fliC-negative EPEC did not elicit a significant IL-8 response despite increased mucosal colonization compared with wild-type EPEC. PMID: 19134113
    11. FliC is secreted by the LEE-encoded type III secretion system and stimulates TLR5 signalling but does not confer motility. PMID: 19200386

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  • 亞細(xì)胞定位：
    Secreted. Bacterial flagellum.
  • 蛋白家族：
    Bacterial flagellin family
  • 數(shù)據(jù)庫鏈接：

    KEGG: ecj:JW1908

    STRING: 316407.1736591