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  4. Recombinant Escherichia coli Ribosomal RNA large subunit methyltransferase N (rlmN)

Recombinant Escherichia coli Ribosomal RNA large subunit methyltransferase N (rlmN)

  • 中文名稱：
    大腸桿菌rlmN重組蛋白
  • 貨號：
    CSB-YP339759ENV
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大腸桿菌rlmN重組蛋白
  • 貨號：
    CSB-EP339759ENV
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    大腸桿菌rlmN重組蛋白
  • 貨號：
    CSB-EP339759ENV-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大腸桿菌rlmN重組蛋白
  • 貨號：
    CSB-BP339759ENV
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大腸桿菌rlmN重組蛋白
  • 貨號：
    CSB-MP339759ENV
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    rlmN
  • Uniprot No.：
  • 別名：
    rlmN; yfgB; b2517; JW2501; Dual-specificity RNA methyltransferase RlmN; EC 2.1.1.192; 23S rRNA; adenine(2503)-C(2))-methyltransferase; 23S rRNA m2A2503 methyltransferase; Ribosomal RNA large subunit methyltransferase N; tRNA; adenine(37)-C(2))-methyltransferase; tRNA m2A37 methyltransferase
  • 種屬：
    Escherichia coli (strain K12)
  • 蛋白長度：
    full length protein
  • 表達區(qū)域：
    1-384
  • 氨基酸序列
    MSEQLVTPEN VTTKDGKINL LDLNRQQMRE FFKDLGEKPF RADQVMKWMY HYCCDNFDEM TDINKVLRGK LKEVAEIRAP EVVEEQRSSD GTIKWAIAVG DQRVETVYIP EDDRATLCVS SQVGCALECK FCSTAQQGFN RNLRVSEIIG QVWRAAKIVG AAKVTGQRPI TNVVMMGMGE PLLNLNNVVP AMEIMLDDFG FGLSKRRVTL STSGVVPALD KLGDMIDVAL AISLHAPNDE IRDEIVPINK KYNIETFLAA VRRYLEKSNA NQGRVTIEYV MLDHVNDGTE HAHQLAELLK DTPCKINLIP WNPFPGAPYG RSSNSRIDRF SKVLMSYGFT TIVRKTRGDD IDAACGQLAG DVIDRTKRTL RKRMQGEAID IKAV
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs. m2A2503 modification seems to play a crucial role in the proofreading step occurring at the peptidyl transferase center and thus would serve to optimize ribosomal fidelity. Unmodified tRNA is not a suitable substrate for RlmN, which suggests that RlmN works in a late step during tRNA maturation.
  • 基因功能參考文獻：
    1. this study reports the x-ray structure of a key intermediate in the RlmN reaction, in which a Cys(118)-->Ala variant of the protein is cross-linked to a tRNA(Glu)substrate through the terminal methylene carbon of a formerly methylcysteinyl residue and C2 of adenosine 37. PMID: 27081063
    2. RlmN methyltransferase is a dual-specificity enzyme that modifies both rRNA and tRNA and controls translational accuracy. PMID: 22891362
    3. Reconstitution of the [4Fe-4S] cluster on wild-type apo RlmN followed by addition of S-adenosylmethionine results in rapid production of S-adenosylhomocysteine and the mCys residue. PMID: 21916495
    4. structure of RlmN and RlmN with SAM show single molecule of SAM coordinates [4Fe-4S] cluster; residue Cys(355) is S-methylated and located proximal to SAM methyl group, suggesting SAM that is involved in the initial methyl transfer binds at the same site PMID: 21527678
    5. SAM enzymes identified that methylate C atoms not inherently nucleophilic via intermediacy of 5'-deoxyadenosyl 5'-radical; determined reaction mechanism targeting sp(2)-hybridized carbons at position 2 and 8 of adenosine in 23S RRNA catalyzed by RlmN, Cfr PMID: 21415317

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  • 亞細胞定位：
    Cytoplasm.
  • 蛋白家族：
    Radical SAM superfamily, RlmN family
  • 數(shù)據(jù)庫鏈接：