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  4. Recombinant Eukaryotic translation initiation factor 4E-1 (ife-1)

Recombinant Eukaryotic translation initiation factor 4E-1 (ife-1)

  • 中文名稱：
    秀麗隱桿線蟲ife-1重組蛋白
  • 貨號：
    CSB-YP524295CXY
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲ife-1重組蛋白
  • 貨號：
    CSB-EP524295CXY
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲ife-1重組蛋白
  • 貨號：
    CSB-EP524295CXY-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲ife-1重組蛋白
  • 貨號：
    CSB-BP524295CXY
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲ife-1重組蛋白
  • 貨號：
    CSB-MP524295CXY
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    ife-1
  • Uniprot No.：
  • 別名：
    ife-1; F53A2.6; Eukaryotic translation initiation factor 4E-1; eIF-4E-1; eIF4E-1; eIF-4F 25 kDa subunit; mRNA cap-binding protein
  • 種屬：
    Caenorhabditis elegans
  • 蛋白長度：
    full length protein
  • 表達區(qū)域：
    1-212
  • 氨基酸序列
    MTETEQTTAP IYPLKRNWTW WYLNDERNKS WEDRLKKVYT FNTVSEFWAL YDAIRPPSGL NALCDYNVFR DDIQPMWEVP ENSNGGRWLI VIDKGKTPEM VDAIWLEILM ALVGEQFGKD MESICGLVCN VRGKGSKISV WTKDCNDDET NMRIGVVLKE KLMAASKDHS KPLFDVIRYE DHESCQKKTS SVVKAKLSLH SSDAPVAEKS AV
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. All 5 eIF4E proteins bind monomethyl cap structures. Only ife-1, ife-2 and ife-5 bind trimethyl cap structures which result from trans-splicing. Translation of trimethyl cap structure mRNAs may be regulated by intracellular redox state; disulfide bonds change the width and depth of the cap-binding cavity determining selectivity to mRNA caps. Required for progression through meiotic divisions during spermatogenesis and for the production of viable sperm. It is not required during oogenesis.
  • 基因功能參考文獻：
    1. Altered MAPK activation in oocytes suggests dual roles for IFE-1, both promoting and suppressing oocyte maturation at different stages. PMID: 26542024
    2. A germline-specific isoform of eIF4E (IFE-1) is required for efficient translation of stored mRNAs and maturation of both oocytes and sperm. PMID: 19383718
  • 亞細胞定位：
    Cytoplasm. Note=When associated with pgl-1.
  • 蛋白家族：
    Eukaryotic initiation factor 4E family
  • 組織特異性：
    Enriched in the germline from L3 larvae to adults; regions of the gonad undergoing spermatogenesis. Expressed in germ granules (P granules); when associated with pgl-1.
  • 數(shù)據庫鏈接：

    STRING: 6239.F53A2.6a

    UniGene: Cel.23053