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  4. Recombinant Mycobacterium tuberculosis Fructose-bisphosphate aldolase (fba)

Recombinant Mycobacterium tuberculosis Fructose-bisphosphate aldolase (fba)

  • 中文名稱：
    結(jié)核分枝桿菌fba重組蛋白
  • 貨號(hào)：
    CSB-YP302296MVZ
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    結(jié)核分枝桿菌fba重組蛋白
  • 貨號(hào)：
    CSB-EP302296MVZ
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    結(jié)核分枝桿菌fba重組蛋白
  • 貨號(hào)：
    CSB-EP302296MVZ-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    結(jié)核分枝桿菌fba重組蛋白
  • 貨號(hào)：
    CSB-BP302296MVZ
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    結(jié)核分枝桿菌fba重組蛋白
  • 貨號(hào)：
    CSB-MP302296MVZ
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    fba
  • Uniprot No.：
  • 種屬：
    Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
  • 蛋白長(zhǎng)度：
    full length protein
  • 表達(dá)區(qū)域：
    1-344
  • 氨基酸序列
    MPIATPEVYAEMLGQAKQNSYAFPAINCTSSETVNAAIKGFADAGSDGIIQFSTGGAEFGSGLGVKDMVTGAVALAEFTHVIAAKYPVNVALHTDHCPKDKLDSYVRPLLAISAQRVSKGGNPLFQSHMWDGSAVPIDENLAIAQELLKAAAAAKIILEIEIGVVGGEEDGVANEINEKLYTSPEDFEKTIEALGAGEHGKYLLAATFGNVHGVYKPGNVKLRPDILAQGQQVAAAKLGLPADAKPFDFVFHGGSGSLKSEIEEALRYGVVKMNVDTDTQYAFTRPIAGHMFTNYDGVLKVDGEVGVKKVYDPRSYLKKAEASMSQRVVQACNDLHCAGKSLTH
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis.
  • 基因功能參考文獻(xiàn)：
    1. In vitro studies of Deltafba revealed that lack of FBA could only be compensated for by a specific balance of glucose and butyrate in which growth and metabolism of butyrate were determined by Mycobacterium tuberculosis's ability to co-catabolize glucose. PMID: 24851864
    2. Determined is the the X-ray structure of the Fba-inhibitor phosphoglycolohydroxamate complex to 1.58 A. PMID: 23298222
    3. Glycolytic and non-glycolytic functions of Mycobacterium tuberculosis fructose-1,6-bisphosphate aldolase, an essential enzyme produced by replicating and non-replicating bacilli. PMID: 21949126
    4. cloning, purification & characterization of fructose 1,6-bisphosphate aldolase; first Class II Type A aldolase found in the tetrameric form & its catalytic efficiency for FBP cleavage is also highest among the Class II A aldolases characterized to date PMID: 15294302
    5. The X-ray structure of MtFBA in complex with its substrates, was determined. PMID: 19167403

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  • 蛋白家族：
    Class II fructose-bisphosphate aldolase family
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: mtu:Rv0363c

    STRING: 83332.Rv0363c