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  4. Recombinant Human Deoxyribonuclease gamma (DNASE1L3)

Recombinant Human Deoxyribonuclease gamma (DNASE1L3)

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  • 中文名稱：
    人DNASE1L3重組蛋白
  • 貨號：
    CSB-EP621686HU
  • 規(guī)格：
    ￥1836
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP621686HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) DNASE1L3.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP621686HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) DNASE1L3.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 別名：
    Deoxyribonuclease gamma; Deoxyribonuclease I like 3; Deoxyribonuclease I like III; Deoxyribonuclease I-like 3; DHP 2; DHP2; DNAS1L3; DNase gamma; DNase I homolog protein 2; DNase I homolog protein DHP2; DNase I like 3; DNase I-like 3; DNASE1L3; DNSL3_HUMAN; Liver and spleen DNase; LS DNase; LS-DNase; LSD; SLEB 16; SLEB16
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    Full Length of Mature Protein
  • 來源：
    E.coli
  • 分子量：
    60.4kDa
  • 表達區(qū)域：
    21-305aa
  • 氨基酸序列
    MRICSFNVRSFGESKQEDKNAMDVIVKVIKRCDIILVMEIKDSNNRICPILMEKLNRNSRRGITYNYVISSRLGRNTYKEQYAFLYKEKLVSVKRSYHYHDYQDGDADVFSREPFVVWFQSPHTAVKDFVIIPLHTTPETSVKEIDELVEVYTDVKHRWKAENFIFMGDFNAGCSYVPKKAWKNIRLRTDPRFVWLIGDQEDTTVKKSTNCAYDRIVLRGQEIVSSVVPKSNSVFDFQKAYKLTEEEALDVSDHFPVEFKLQSSRAFTNSKKSVTLRKKTKSKRS
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標簽：
    N-terminal GST-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
    Note: If you have any special requirement for the glycerol content, please remark when you place the order.
    If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    3-7 business days
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產(chǎn)品描述：
        脫氧核糖核酸酶gamma (DNASE1L3)能夠同時切割單鏈和雙鏈DNA，產(chǎn)生含有5'-磷酸基團和3'-羥基端的單脫氧核苷酸和寡脫氧核苷酸。在人體中，DNASE1L3主要參與到細胞的內(nèi)部機制中，包括細胞凋亡和壞死過程中的DNA片段切割4。此外，它還能在細胞培養(yǎng)過程中作為添加物來消化細胞間的粘連，在細胞凋亡TUNEL檢測中部分剪切基因組DNA作為陽性對照等。
        在科研領(lǐng)域，DNASE1L3也是一個重要的研究對象。由于其在DNA切割方面的特性，使得它在許多分子生物學和遺傳學的實驗中得到了廣泛的應用。例如，在RNA提取或反轉(zhuǎn)錄前去除gDNA，或者在rRNA去除中消化DNA探針，缺口平移進行DNA標記，足跡法分析DNA-蛋白質(zhì)相互作用，生成隨機的DNA文庫，減少細胞裂解物或蛋白提取物的粘性等。
        華美生物大腸桿菌表達系統(tǒng)表達了全長重組人脫氧核糖核酸酶gamma (DNASE1L3)，助您進行相關(guān)研究。
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Has DNA hydrolytic activity. Is capable of both single- and double-stranded DNA cleavage, producing DNA fragments with 3'-OH ends. Can cleave chromatin to nucleosomal units and cleaves nucleosomal and liposome-coated DNA. Acts in internucleosomal DNA fragmentation (INDF) during apoptosis and necrosis. The role in apoptosis includes myogenic and neuronal differentiation, and BCR-mediated clonal deletion of self-reactive B cells. Is active on chromatin in apoptotic cell-derived membrane-coated microparticles and thus suppresses anti-DNA autoimmunity. Together with DNASE1, plays a key role in degrading neutrophil extracellular traps (NETs). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation. Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation.
  • 基因功能參考文獻：
    1. The authors found a significant reduction in serum DNase1l3 level in dermatomyositis/polymyositis and systemic lupus erythematosus, which may associate with clinic features and disease activity. PMID: 28039554
    2. Data indicate a likely functional variant influencing scleroderma susceptibility at deoxyribonuclease I-like 3 protein DNASE1L3 missense polymorphism rs35677470. PMID: 25332064
    3. Single nucleotide polymorphisms in the DNASE1L3 is associated with autoimmune diseases. PMID: 24206041
    4. DNASE1L3 mutations occur in hypocomplementemic urticarial vasculitis syndrome. PMID: 23666765
    5. We identified a rare autosomal recessive form of systemic lupus erythematosus, in which autozygome analysis revealed a null mutation in the DNASE1L3 gene PMID: 22019780
    6. investigates the distribution of DNASE1L3 gene SNPs in exons of the gene in eight Asian, three African, and three Caucasian populations worldwide using newly devised genotyping methods. PMID: 21692081
    7. The Poly(ADP-ribose) polymerase-1-regulated endonuclease DNAS1L3 is required for etoposide-induced internucleosomal DNA fragmentation and increases etoposide cytotoxicity in transfected osteosarcoma cells. PMID: 12154052
    8. Identification of two functional nuclear localization signals in DNase gamma that have a role in apoptotic DNA fragmentation. PMID: 12943533
    9. DNase gamma is involved in the generation of resected double-strand DNA breaks associated with somatic hypermutation PMID: 15629432
    10. DNAS1L3 plays an active role in lymphoma cell sensitization to VP-16 and its deficiency may constitute a novel mechanism of drug resistance in these cells PMID: 16427601
    11. A Caucasian-specific allele in SNP R206C produces an inactive form of DNase Il3. PMID: 19559017
    12. Results distinguish DNase gamma-dependent and CAD/DFF40-dependent DNA fragmentations, and show that even if DNA fragmentation is initiated by CAD/DFF40, DNase gamma is required for the more complete digestion of the genomic DNA in dying cells. PMID: 19574717

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  • 相關(guān)疾?。?/div>
    Systemic lupus erythematosus 16 (SLEB16)
  • 亞細胞定位：
    Nucleus. Endoplasmic reticulum. Secreted.
  • 蛋白家族：
    DNase I family
  • 組織特異性：
    Liver and spleen.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 2959

    OMIM: 602244

    KEGG: hsa:1776

    STRING: 9606.ENSP00000316193

    UniGene: Hs.476453