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  4. Recombinant Human Protein NDNF (NDNF)

Recombinant Human Protein NDNF (NDNF)

  • 中文名稱：
    人NDNF重組蛋白
  • 貨號：
    CSB-YP823447HU
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    人NDNF重組蛋白
  • 貨號：
    CSB-EP823447HU
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    人NDNF重組蛋白
  • 貨號：
    CSB-EP823447HU-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    人NDNF重組蛋白
  • 貨號：
    CSB-BP823447HU
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    人NDNF重組蛋白
  • 貨號：
    CSB-MP823447HU
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    NDNF
  • Uniprot No.：
  • 別名：
    C4orf31; Fibronectin type III domain containing protein C4orf31; NDNF; NDNF_HUMAN; Neuron-derived neurotrophic factor; PRO6487; Protein NDNF; UNQ2748
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達(dá)區(qū)域：
    20-568
  • 氨基酸序列
    Q KLPTRDEELF QMQIRDKAFF HDSSVIPDGA EISSYLFRDT PKRYFFVVEE DNTPLSVTVT PCDAPLEWKL SLQELPEDRS GEGSGDLEPL EQQKQQIINE EGTELFSYKG NDVEYFISSS SPSGLYQLDL LSTEKDTHFK VYATTTPESD QPYPELPYDP RVDVTSLGRT TVTLAWKPSP TASLLKQPIQ YCVVINKEHN FKSLCAVEAK LSADDAFMMA PKPGLDFSPF DFAHFGFPSD NSGKERSFQA KPSPKLGRHV YSRPKVDIQK ICIGNKNIFT VSDLKPDTQY YFDVFVVNIN SNMSTAYVGT FARTKEEAKQ KTVELKDGKI TDVFVKRKGA KFLRFAPVSS HQKVTFFIHS CLDAVQIQVR RDGKLLLSQN VEGIQQFQLR GKPKAKYLVR LKGNKKGASM LKILATTRPT KQSFPSLPED TRIKAFDKLR TCSSATVAWL GTQERNKFCI YKKEVDDNYN EDQKKREQNQ CLGPDIRKKS EKVLCKYFHS QNLQKAVTTE TIKGLQPGKS YLLDVYVIGH GGHSVKYQSK VVKTRKFC
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產(chǎn)品描述：
    重組人源NDNF（NADH脫氫酶復(fù)合物裝配因子）蛋白，它在細(xì)胞內(nèi)的功能與線粒體呼吸鏈復(fù)合物的裝配和維護(hù)有關(guān)。下面是重組人NDNF的一般步驟：
    設(shè)計和合成基因：基于人NDNF的氨基酸序列，設(shè)計并合成含有NDNF基因的DNA序列。這可以通過基因合成公司或PCR方法完成。
    克隆基因：將NDNF基因插入到表達(dá)載體中，這可以通過PCR擴增和限制性內(nèi)切酶切割的方法完成。建議選擇一些經(jīng)過驗證的表達(dá)載體，如pET系列載體，這些載體中包含了一些促進(jìn)蛋白表達(dá)的元件，如啟動子、增強子、終止子等。
    轉(zhuǎn)染宿主細(xì)胞：在細(xì)胞中轉(zhuǎn)染含有NDNF基因的表達(dá)載體。這些宿主細(xì)胞可以選擇常見的哺乳動物細(xì)胞系，如CHO細(xì)胞、HEK293細(xì)胞等。
    培養(yǎng)和表達(dá)蛋白：根據(jù)表達(dá)載體中的啟動子和調(diào)節(jié)元件，誘導(dǎo)NDNF蛋白在轉(zhuǎn)染的宿主細(xì)胞中表達(dá)。在培養(yǎng)期間，需要添加抗生素等來保持表達(dá)載體的穩(wěn)定。
    蛋白質(zhì)的純化：通過細(xì)胞破碎和離心等方法提取含有NDNF蛋白的細(xì)胞裂解液。然后使用親和層析、離子交換層析、凝膠過濾層析等方法對蛋白進(jìn)行純化。
    蛋白質(zhì)的鑒定和活性檢測：使用SDS-PAGE、Western blot等方法對純化后的NDNF蛋白進(jìn)行鑒定和定量。此外，可以使用酶活性分析等方法來檢測其生物學(xué)活性和功能。
    需要注意的是，線粒體蛋白的表達(dá)和純化需要特殊的條件和方法。因此，在進(jìn)行NDNF的表達(dá)和純化時，需要注意線粒體蛋白的穩(wěn)定性和功能性，避免蛋白質(zhì)的降解和失活。
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Secretory protein that plays a role in various cellular processes. Acts as a chemorepellent acting on gonadotropin-releasing hormone (GnRH) expressing neurons regulating their migration to the hypothalamus. Also promotes neuron migration, growth and survival as well as neurite outgrowth and is involved in the development of the olfactory system. May also act through the regulation of growth factors activity and downstream signaling. Also regulates extracellular matrix assembly and cell adhesiveness. Promotes endothelial cell survival, vessel formation and plays an important role in the process of revascularization through NOS3-dependent mechanisms.
  • 基因功能參考文獻(xiàn)：
    1. NDNF acts as an endogenous modulator that promotes endothelial cell function and ischemia-induced revascularization through eNOS-dependent mechanisms PMID: 24706764
    2. direct duplication of chromosome 4q27q31.3 de novo results in partial trisomy of its long arm. PMID: 16970039
  • 亞細(xì)胞定位：
    Secreted.
  • 組織特異性：
    Expressed in neurons along the gonadotropin-releasing hormone (GnRH) expressing neurons migratory route.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 26256

    OMIM: 616506

    KEGG: hsa:79625

    STRING: 9606.ENSP00000369014

    UniGene: Hs.709520