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  4. Recombinant Kinetochore protein ndc-80 (ndc-80), partial

Recombinant Kinetochore protein ndc-80 (ndc-80), partial

  • 中文名稱：
    秀麗隱桿線蟲ndc-80重組蛋白
  • 貨號：
    CSB-YP613757CXY
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲ndc-80重組蛋白
  • 貨號：
    CSB-EP613757CXY
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲ndc-80重組蛋白
  • 貨號：
    CSB-EP613757CXY-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲ndc-80重組蛋白
  • 貨號：
    CSB-BP613757CXY
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲ndc-80重組蛋白
  • 貨號：
    CSB-MP613757CXY
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    ndc-80
  • Uniprot No.：
  • 別名：
    ndc-80; W01B6.9Kinetochore protein ndc-80; CeNDC-80; Hec1 homolog
  • 種屬：
    Caenorhabditis elegans
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Acts as a component of the essential kinetochore-associated ndc-80 complex, which is required for chromosome segregation in mitosis and meiosis and spindle checkpoint activity. Plays a role in kinetochore assembly and recruits the checkpoint protein mdf-2 and the spindly-like protein spdl-1 to unattached kinetochores. Mediates the formation of end-on kinetochore-microtubule attachments through recruitment of spdl-1. The ndc-80 complex synergistically enhances the affinity of the ska-1 complex for microtubules and may allow the ndc-80 complex to track depolymerizing microtubules.
  • 基因功能參考文獻(xiàn)：
    1. Ndc80-tail dephosphorylation promotes stabilization of kinetochore-microtubule attachments via the Ska complex. PMID: 28535376
    2. Ndc80 complex bound to microtubules binds more tightly to beta-tubulin. PMID: 26941333
    3. KLP-7 acts through the Ndc80 complex to limit pole number in C. elegans oocyte meiotic spindle assembly. PMID: 26370499
    4. kinetochore dynein module inhibited microtubule binding by the Ndc80 complex; kinetochore dynein module directly regulated Ndc80, independently of phosphorylation by Aurora B kinase, and this regulation was required for accurate segregation PMID: 24231804
  • 亞細(xì)胞定位：
    Nucleus. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton.
  • 蛋白家族：
    NDC80/HEC1 family
  • 數(shù)據(jù)庫鏈接：

    KEGG: cel:CELE_W01B6.9

    STRING: 6239.W01B6.9.1

    UniGene: Cel.12240