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  4. Recombinant Lissencephaly-1 homolog (lis-1)

Recombinant Lissencephaly-1 homolog (lis-1)

  • 中文名稱：
    秀麗隱桿線蟲lis-1重組蛋白
  • 貨號：
    CSB-YP873586CXY
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲lis-1重組蛋白
  • 貨號：
    CSB-EP873586CXY
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲lis-1重組蛋白
  • 貨號：
    CSB-EP873586CXY-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲lis-1重組蛋白
  • 貨號：
    CSB-BP873586CXY
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲lis-1重組蛋白
  • 貨號：
    CSB-MP873586CXY
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    lis-1
  • Uniprot No.：
  • 別名：
    lis-1; pnm-1; T03F6.5; Lissencephaly-1 homolog; Pronuclear migration abnormal protein 1
  • 種屬：
    Caenorhabditis elegans
  • 蛋白長度：
    full length protein
  • 表達區(qū)域：
    1-404
  • 氨基酸序列
    MSLSERQKEE INRAIAEYMQ NNGYSESFSV FLKESSLSEN DIKPLGGILE KKWTTVLRLQ RKVNDLESKL QESQREINHG APTRDKRQAA DWIPRPPETQ KLTGHRLPIT RVIFHPLWTI MASCSEDATI KVWDYETGQL ERTLKGHTDA VNDIAIDAAG KQLVSCSSDL SIKLWDFGQT YDCLKSLKGH EHTVSSVTFL PTGDFVLSAS RDHTIKQWDI STGYCVYTFR GHNDWVRMIR ISNDGTLFAS ASLDQTVTVW SFATKSAKLV LRDHEHAVEC VEWAPDTAYT NVTGQQPEGN STHILFSGSR DRSIKAWNIN TGDVLFTLLA HENWVRGLAF HPKGKYLISV ADDKTLRVWE LSAQRCMKAI EAHEHFVSTV AFHQTSPFVI TGSVDMSCKV WECR
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus end. Required for several dynein- and microtubule-dependent processes such as nuclear migration during cell division. Part of a complex with nud-2, which is recruited to the nuclear envelope by unc-83, where, in turn, it recruits dynein to the nuclear surface and regulates nuclear migration in hypodermal precursor cells. Plays a role in GABAergic synaptic vesicle localization in the ventral nerve cord. Required for neuronal cell differentiation.
  • 基因功能參考文獻：
    1. The convulsions mimicking epilepsy can be induced by a mutation in a C. elegans lis-1 allele (pnm-1), in combination with a chemical antagonist of gamma-aminobutyric acid (GABA) neurotransmitter signaling. PMID: 15254012
    2. Dynein and Lis1, albeit functioning in identical processes, are targeted partially independently of one another. PMID: 15331665
    3. lis-1 induces a spindle checkpoint-dependent mitotic arrest in the germline and an increase in germ-cell apoptosis. PMID: 17376425
  • 亞細胞定位：
    Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Chromosome, centromere, kinetochore. Nucleus envelope.
  • 蛋白家族：
    WD repeat LIS1/nudF family
  • 組織特異性：
    Expressed in all classes of neurons in the ventral cord. Expressed in the multinucleate spermathecal valves and adult seam cells.
  • 數據庫鏈接：

    KEGG: cel:CELE_T03F6.5

    STRING: 6239.T03F6.5

    UniGene: Cel.19492