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  4. Recombinant Methanosarcina mazei RNA 3'-terminal phosphate cyclase (rtcA)

Recombinant Methanosarcina mazei RNA 3'-terminal phosphate cyclase (rtcA)

  • 中文名稱(chēng)：
    馬澤氏甲烷八疊球菌rtcA重組蛋白
  • 貨號(hào)：
    CSB-YP819109MSN
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱(chēng)：
    馬澤氏甲烷八疊球菌rtcA重組蛋白
  • 貨號(hào)：
    CSB-EP819109MSN
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱(chēng)：
    馬澤氏甲烷八疊球菌rtcA重組蛋白
  • 貨號(hào)：
    CSB-EP819109MSN-B
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱(chēng)：
    馬澤氏甲烷八疊球菌rtcA重組蛋白
  • 貨號(hào)：
    CSB-BP819109MSN
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱(chēng)：
    馬澤氏甲烷八疊球菌rtcA重組蛋白
  • 貨號(hào)：
    CSB-MP819109MSN
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    rtcA
  • Uniprot No.：
  • 別名：
    rtcA; MM_1297; RNA 3'-terminal phosphate cyclase; RNA cyclase; RNA-3'-phosphate cyclase; EC 6.5.1.4
  • 種屬：
    Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
  • 蛋白長(zhǎng)度：
    full length protein
  • 表達(dá)區(qū)域：
    1-352
  • 氨基酸序列
    MIEIDGSYGE GGGQLVRTAV ALSAVTGEEI KVTNIRENRP NPGLKQQHLK ALETAAKICG ARVSGLSQGS SELFFAPVEI KGGKYNIDIG TAGSITLLLQ CIMPALPFVE EKVELTIRGG TDVAWSPTID YLKHVTLRAL EQFGYAGSVT LKERGYYPRG GGSVSAVFEP CKLHGFHFRK TEGNKKTESN PVGEGLKGEK SMMEEIIGVS HASNLPAHVP ARQAESARSL FLEAGYDARI DVSSSEFFST GSGITLWKGY CGGSALGKKG LPAEKVGRQA AEEVIKELRA GAAVDIHLAD QLIPYMALAG NSSFTVRELT LHAATNIWVT EQFLDVKFKI KEKEGLFEVS VG
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.
  • 亞細(xì)胞定位：
    Cytoplasm.
  • 蛋白家族：
    RNA 3'-terminal cyclase family, Type 1 subfamily
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: mma:MM_1297

    STRING: 192952.MM_1297