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  4. Recombinant Mouse N-acetylglucosaminyl-phosphatidylinositol biosynthetic protein (Piga), partial

Recombinant Mouse N-acetylglucosaminyl-phosphatidylinositol biosynthetic protein (Piga), partial

  • 中文名稱：
    小鼠Piga重組蛋白
  • 貨號：
    CSB-YP714486MO
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Piga重組蛋白
  • 貨號：
    CSB-EP714486MO
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Piga重組蛋白
  • 貨號：
    CSB-EP714486MO-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Piga重組蛋白
  • 貨號：
    CSB-BP714486MO
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Piga重組蛋白
  • 貨號：
    CSB-MP714486MO
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Piga
  • Uniprot No.：
  • 別名：
    Piga; N-acetylglucosaminyl-phosphatidylinositol biosynthetic protein; GlcNAc-PI synthesis protein; Phosphatidylinositol-glycan biosynthesis class A protein; PIG-A
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Catalytic subunit of the glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex that catalyzes the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol and participates in the first step of GPI biosynthesis.
  • 基因功能參考文獻(xiàn)：
    1. The reliability of using GPI anchor-deficiency as a valid reporter of Pig-a gene mutation. PMID: 29115020
    2. The assay may be useful for mutagenicity screening of environmental agents as well as for testing hypotheses in vitro before committing to in vivo Pig-a assays. PMID: 29098723
    3. Pig-a mutations were identified in all clones developed from CD90-deficient cells; no Pig-a mutations were found in clones of CD90-proficient cells. The spectrum of B[a]P-induced Pig-a mutations was dominated by basepair substitutions, small insertions and deletions at G:C, or at sequences rich in G:C content. PMID: 29150045
    4. Diet-induced obesity increases the frequency of Pig-a mutant erythrocytes in male C57BL/6J mice. PMID: 27739633
    5. Reduced IgE/antigen-mediated passive cutaneous anaphylaxis is detected in mice with Piga-deficient mast cells. PMID: 21865342
    6. Deletion of the Pig-a gene in hematopoietic cells does not cause frank marrow failure but leads to the appearance of clonally-restricted, inactive yet functionally competent CD8 T cells. PMID: 19679885
    7. Piga gene mutation does not alter susceptibility to cell death in thymocytes, granulocytes, and hematopoietic progenitor cells. PMID: 12488505
    8. Pig-a-knockout females are infertile, and eggs recovered from the females after mating are unfertilized. PMID: 12692150
    9. Lack of this protein does not cause a retention of progenitor cells in the bone marrow. PMID: 15297852
    10. processing profilaggrin to its monomeric form was impaired in Pig-a null mouse epidermis PMID: 15304084
    11. Piga expression is required to properly orient hair cells in the inner ear, a process regulated by the planar cell polarity pathway. PMID: 19762422

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  • 亞細(xì)胞定位：
    Endoplasmic reticulum membrane; Single-pass membrane protein.
  • 蛋白家族：
    Glycosyltransferase group 1 family, Glycosyltransferase 4 subfamily
  • 數(shù)據(jù)庫鏈接：