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  4. Recombinant Nuclear migration and anchoring protein unc-84 (unc-84), partial

Recombinant Nuclear migration and anchoring protein unc-84 (unc-84), partial

  • 中文名稱：
    秀麗隱桿線蟲unc-84重組蛋白
  • 貨號：
    CSB-YP636339CXY
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲unc-84重組蛋白
  • 貨號：
    CSB-EP636339CXY
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲unc-84重組蛋白
  • 貨號：
    CSB-EP636339CXY-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲unc-84重組蛋白
  • 貨號：
    CSB-BP636339CXY
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲unc-84重組蛋白
  • 貨號：
    CSB-MP636339CXY
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    unc-84
  • Uniprot No.：
  • 別名：
    unc-84; F54B11.3Nuclear migration and anchoring protein unc-84; Uncoordinated protein 84
  • 種屬：
    Caenorhabditis elegans
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Involved in nuclear migration and anchoring in hypodermal precursor cells. Most likely recruits anc-1 to the nuclear envelope where anc-1 functions to tether the nucleus to the actin cytoskeleton. Component of the unc-83-unc-84 LINC (LInker of Nucleoskeleton and Cytoskeleton) complex where it recruits and interacts with unc-83 to form a bridge connecting the nuclear envelope to the cytoskeleton which allows for nuclear transport along microtubules. Its role in nuclear migration may be in association with lamin, lmn-1. Regulates nuclear migrations in one-cell embryos, controlling the posterior migration of the male pronucleus following fertilization. Not required for centrosome attachment to the nucleus. Plays a role in the maintenance of the nuclear envelope architecture in body wall muscle cells. May be involved in DNA damage repair through an association with zyg-12. Potentially has roles in homologous recombination, double strand break repair and meiotic recombination. Specifically, may in part inhibit non-homologous end joining repair, most likely through recruiting fan-1 to the nucleoplasm, to facilitate the repair of DNA cross-links.
  • 基因功能參考文獻：
    1. SUN-KASH bridges are only required to maintain nuclear envelope spacing in cells subjected to increased mechanical forces. PMID: 25023515
    2. targeting UNC-84 requires an initial step that actively transports UNC-84 from the peripheral endoplasmic reticulum to the nuclear envelope PMID: 21411627
    3. UNC-84 and UNC-83, the KASH and SUN domain proteins, bridge the nuclear envelope, connecting the nuclear lamina to cytoskeletal components. PMID: 16481402
  • 亞細胞定位：
    Nucleus inner membrane; Single-pass type II membrane protein. Cytoplasm, cytoskeleton.
  • 組織特異性：
    Expressed in all somatic cells. Not expressed in germ cells in the mitotic and transition zones of the gonad. One study shows expression at the beginning of the late pachytene stage in the proximal gonad, but there is no expression in the male germline, s
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