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  4. Recombinant Poly (A) RNA polymerase gld-2, partial

Recombinant Poly (A) RNA polymerase gld-2, partial

  • 中文名稱：
    秀麗隱桿線蟲gld-2重組蛋白
  • 貨號：
    CSB-YP515704CXY
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲gld-2重組蛋白
  • 貨號：
    CSB-EP515704CXY
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲gld-2重組蛋白
  • 貨號：
    CSB-EP515704CXY-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲gld-2重組蛋白
  • 貨號：
    CSB-BP515704CXY
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲gld-2重組蛋白
  • 貨號：
    CSB-MP515704CXY
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    gld-2
  • Uniprot No.：
  • 別名：
    gld-2; ZC308.1; Poly(A) RNA polymerase gld-2; EC 2.7.7.19; Defective in germ line development protein 2
  • 種屬：
    Caenorhabditis elegans
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. Acts as a regulator of mitosis/meiosis required for progression through meiotic prophase during oogenesis and spermatogenesis and for promotion of the entry into meiosis from the mitotic cell cycle. May act by regulating and activating gld-1 mRNA activity in germline.
  • 基因功能參考文獻：
    1. investigate whether the functions of the cytoplasmic poly(A) polymerase (cytoPAP) GLD-2 in C. elegans germ cells exclusively depend on its catalytic activity; findings highlight the enzymatic importance of noncanonical poly(A) polymerases and emphasize the pivotal role of poly(A) tail-centered cytoplasmic mRNA regulation in germ cell biology. PMID: 28490506
    2. GLD-2 thus appears to have evolved specialized nucleotidyl-transferase properties that match the 3' end features of dormant cytoplasmic mRNAs PMID: 26124149
    3. The analyses suggest that GLD-2 activity mediates mRNA stability of many translationally repressed mRNAs. PMID: 25217583
    4. Data suggest that the FBF-2/GLD-2 protein directed polyadenylation of the endogenous mRNA, compensating for that mRNA's natural deadenylation. PMID: 21911377
    5. Regulates Gld-1 messenger RNA in germ cells, enabling the transition from mitosis to meiosis. PMID: 17012378
    6. FBF can affect polyadenylation either negatively by its CCF-1 interaction or positively by its GLD-2 interaction in control of GLD1 expression. PMID: 19221201
    7. GLD-4 is predominantly expressed in germ cells, and its activity is essential for early meiotic progression of male and female gametes in the absence of GLD-2 PMID: 19339688

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  • 亞細胞定位：
    Cytoplasm.
  • 蛋白家族：
    DNA polymerase type-B-like family, GLD2 subfamily
  • 組織特異性：
    Germline-specific.
  • 數據庫鏈接：

    KEGG: cel:CELE_ZC308.1

    STRING: 6239.ZC308.1a

    UniGene: Cel.9280