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  4. Recombinant Pyrococcus furiosus Coenzyme A disulfide reductase (PF1186)

Recombinant Pyrococcus furiosus Coenzyme A disulfide reductase (PF1186)

  • 中文名稱：
    毛球菌PF1186重組蛋白
  • 貨號(hào)：
    CSB-YP823622FHW
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱：
    毛球菌PF1186重組蛋白
  • 貨號(hào)：
    CSB-EP823622FHW
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱：
    毛球菌PF1186重組蛋白
  • 貨號(hào)：
    CSB-EP823622FHW-B
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    毛球菌PF1186重組蛋白
  • 貨號(hào)：
    CSB-BP823622FHW
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱：
    毛球菌PF1186重組蛋白
  • 貨號(hào)：
    CSB-MP823622FHW
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    PF1186
  • Uniprot No.：
  • 別名：
    PF1186Coenzyme A disulfide reductase; CoA-disulfide reductase; CoADR; EC 1.8.1.14
  • 種屬：
    Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
  • 蛋白長(zhǎng)度：
    full length protein
  • 表達(dá)區(qū)域：
    1-442
  • 氨基酸序列
    MEKKKVVIIG GGAAGMSAAS RVKRLRPEWD VKVFEATEWV SHAPCGIPYV VEGIAPKEKL MHYPPEVFIK KRGIDLHLKA EVIEVETGYV RVRENGEEKS YEWDYLVFAN GASPQIPEIE GVDLPGVFTA DLPPDAVAIT EYMEKNKVED VVIIGTGYIA LEMAEAFVTR GKNVTLIGRS ERVLRKTFDK EITDIVEEKL RQHLNLRLHE KTLSIEGRER VEKVITDGGE YKADLVIIAT GIKPNVELAK QLGVKIGETG AIWTNEKMQT SVENVYAAGD VAETKHVITG KRVWIPLAPA GNKMGYVAGS NIAGKEIEFP GVLGTSITKF MDLEIGKTGL TENEAVKEGY DVRTAFIKAN TKPHYYPGGR EIWLKGVVDN ETNRLLGVQA VGAEILPRID SAAAMITAGF TTKDVFFTDL AYAPPFAPVW DPLIVLARVL KF
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Catalyzes the CoA-dependent reduction of elemental sulfur (S(0)) to produce hydrogen sulfide. Can use both NADPH and NADH, but shows a preference for NADPH. May enable S(0) to be used, via sulfide, for iron-sulfur cluster synthesis by SipA (Probable). Also shows coenzyme A disulfide reductase (CoADR) activity with both NADH and NADPH. However, CoADR specific activity is about 20-fold lower than the sulfur reduction assay and CoADR activity appears to be an artifactual side reaction and is not thought to have any physiological relevance. Also shows NAD(P)H oxidase activity with both NADH and NADPH.
  • 基因功能參考文獻(xiàn)：
    1. The enzyme encoded by PF1186 is a NAD(P)H elemental sulfur oxidoreductase (NSR). PMID: 17449625
  • 亞細(xì)胞定位：
    Cytoplasm.
  • 蛋白家族：
    Class-III pyridine nucleotide-disulfide oxidoreductase family
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: pfu:PF1186

    STRING: 186497.PF1186