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  4. Recombinant Saccharomyces cerevisiae Monothiol glutaredoxin-6 (GRX6)

Recombinant Saccharomyces cerevisiae Monothiol glutaredoxin-6 (GRX6)

  • 中文名稱：
    釀酒酵母GRX6重組蛋白
  • 貨號(hào)：
    CSB-YP618601SVG
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母GRX6重組蛋白
  • 貨號(hào)：
    CSB-EP618601SVG
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母GRX6重組蛋白
  • 貨號(hào)：
    CSB-EP618601SVG-B
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母GRX6重組蛋白
  • 貨號(hào)：
    CSB-BP618601SVG
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母GRX6重組蛋白
  • 貨號(hào)：
    CSB-MP618601SVG
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    GRX6
  • Uniprot No.：
  • 別名：
    GRX6; YDL010W; D2890Monothiol glutaredoxin-6
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長(zhǎng)度：
    Full Length of Mature Protein
  • 表達(dá)區(qū)域：
    30-231
  • 氨基酸序列
    N FLTVEIKEET SKAFSTNMDN MAGGSSREYA AMPTSTTNKG SSEVDEEINE IKQKVGLQQP IASVDDSLSA IKNDKGSRIT KAFNVQKEYS LILDLSPIII FSKSTCSYSK GMKELLENEY QFIPNYYIIE LDKHGHGEEL QEYIKLVTGR GTVPNLLVNG VSRGGNEEIK KLHTQGKLLE SLQVWSDGKF SVEQREKPSN N
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 基因功能參考文獻(xiàn)：
    1. Crystal structure of Grx6 in complex with a glutathione-coordinated [2Fe-2S] cluster has been presented. PMID: 27710937
    2. A specific function influenced by Grx6 activity is the homeostasis of intracellular calcium. Grx6-deficient mutants have reduced levels of calcium in the ER lumen, whereas accumulation occurs at the cytosol from extracellular sources. PMID: 25355945
    3. Compared to the classic dithiol glutaredoxin Grx1, Grx6 has a lower glutathione disulfide reductase activity but a higher glutathione S-transferase activity. PMID: 20347849
    4. S. pombe cells harboring plasmid pFGRX6 containing the Grx6 gene had about 1.3-fold elevated Grx activity in the exponential phase, and grew better than the control cells under some stressful conditions. PMID: 18182845
    5. Grx6 and Grx7 do not play a general role in the oxidative folding of proteins in the early secretory pathway but rather counteract the oxidation of specific thiol groups in substrate proteins. PMID: 18400945
    6. GRX6 could be responsible for the regulation of the sulfhydryl oxidative state at the oxidant conditions of the early secretory pathway vesicles. PMID: 18503006

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  • 亞細(xì)胞定位：
    Vacuole.
  • 蛋白家族：
    Glutaredoxin family, Monothiol subfamily
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: sce:YDL010W

    STRING: 4932.YDL010W