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  4. Recombinant Saccharomyces cerevisiae Neutral trehalase (NTH1), partial

Recombinant Saccharomyces cerevisiae Neutral trehalase (NTH1), partial

  • 中文名稱：
    釀酒酵母NTH1重組蛋白
  • 貨號：
    CSB-EP330157SVG
  • 規(guī)格：
    ￥2328
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    NTH1
  • Uniprot No.：
  • 別名：
    NTH1; NTH; YDR001C; YD8119.07C; Neutral trehalase; EC 3.2.1.28; Alpha,alpha-trehalase; Alpha,alpha-trehalose glucohydrolase
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 來源：
    E.coli
  • 分子量：
    27.6kDa
  • 表達(dá)區(qū)域：
    546-751aa
  • 氨基酸序列
    YMWDDESGFFFDYNTKIKHRTSYESATTFWALWAGLATKEQAQKMVEKALPKLEMLGGLAACTERSRGPISISRPIRQWDYPFGWAPHQILAWEGLRSYGYLTVTNRLAYRWLFMMTKAFVDYNGIVVEKYDVTRGTDPHRVEAEYGNQGADFKGAATEGFGWVNASYILGLKYMNSHARRALGACIPPISFFSSLRPQERNLYGL
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標(biāo)簽：
    N-terminal 6xHis-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
    Note: If you have any special requirement for the glycerol content, please remark when you place the order.
    If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Hydrolyzes intracellular trehalose to glucose (Probable). The disaccharide trehalose serves as a storage carbohydrate that is mobilized during nutrient stress (Probable). Regulates the level of trehalose as a protectant for cell integrity during heat stress.
  • 基因功能參考文獻(xiàn)：
    1. EF-hand like motif-containing domain functions as the intermediary through which the 14-3-3 protein modulates the function of the catalytic domain of Nth1 PMID: 24713696
    2. 14-3-3 proteins are required for both activation of trehalase and phosphorylation of the ser21 and ser83 sites. PMID: 23155055
    3. Gene disruption of NTH1 and ATH1 increased tolerance to freezing, heat, high-sugar and ethanol concentration. PMID: 19160808
    4. The behaviour of trehalase mutant strains demonstrates the participation of trehalases Nth1p, Nth2p and Ath1p in the mobilization of intracellular trehalose during growth recovery after saline stress. PMID: 19520725
  • 亞細(xì)胞定位：
    Cytoplasm.
  • 蛋白家族：
    Glycosyl hydrolase 37 family
  • 數(shù)據(jù)庫鏈接：

    KEGG: sce:YDR001C

    STRING: 4932.YDR001C