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  4. Recombinant Saccharomyces cerevisiae P-type cation-transporting ATPase (PCA1), partial

Recombinant Saccharomyces cerevisiae P-type cation-transporting ATPase (PCA1), partial

  • 中文名稱：
    釀酒酵母PCA1重組蛋白
  • 貨號(hào)：
    CSB-YP330870SVG
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母PCA1重組蛋白
  • 貨號(hào)：
    CSB-EP330870SVG
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母PCA1重組蛋白
  • 貨號(hào)：
    CSB-EP330870SVG-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母PCA1重組蛋白
  • 貨號(hào)：
    CSB-BP330870SVG
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母PCA1重組蛋白
  • 貨號(hào)：
    CSB-MP330870SVG
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    PCA1
  • Uniprot No.：
  • 別名：
    PCA1; CAD2; PAY2; YBR295W; YBR2112; P-type cation-transporting ATPase; EC 7.2.2.21; Cadmium resistance protein 2; Cadmium-translocating P-type ATPase; Cd(2+)-exporting ATPase
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Cadmium transporting P-type ATPase which plays a critical role in cadmium resistance by extruding intracellular cadmium. Capable of high affinity copper ion binding, but not active copper ion transport. May play a role in copper resistance by chelating and sequestering copper ions.
  • 基因功能參考文獻(xiàn)：
    1. Determined the roles for the molecular factors of ERAD in dislodging Pca1p from the endoplasmic reticulum (ER). Inactivation of the 20S proteasome leads to accumulation of ubiquitinated Pca1p in the ER membrane, suggesting a role for the proteasome in extraction of Pca1p from the ER. PMID: 27226596
    2. Data indicate that specific cysteine residues in a degron of cation-transporting P-type ATPase PCA1 (Pca1p) sense cadmium. PMID: 27059957
    3. cadmium resistance is primarily caused by an enhanced function of a metal efflux pump, PCA1. PMID: 21483812
    4. Overexpression or deletion of the functional allele in yeast demonstrates that PCA1 is a cadmium efflux pump PMID: 17107946
    5. Cadmium sensing by cysteine residues within this domain circumvents ubiquitination and degradation of Pca1 PMID: 18753133
    6. Direct cadmium sensing at the endoplasmic reticulum by a degron in Pca1 leads to an escape of Pca1 from endoplasmic reticulum-associated degradation. PMID: 19515821

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  • 亞細(xì)胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Cation transport ATPase (P-type) (TC 3.A.3) family, Type IB subfamily
  • 數(shù)據(jù)庫鏈接：

    KEGG: sce:YBR295W

    STRING: 4932.YBR295W