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  4. Recombinant Schizosaccharomyces pombe GTP-binding protein ypt7 (ypt7)

Recombinant Schizosaccharomyces pombe GTP-binding protein ypt7 (ypt7)

  • 中文名稱：
    龐貝裂殖酵母ypt7重組蛋白
  • 貨號(hào)：
    CSB-YP529717SXV
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母ypt7重組蛋白
  • 貨號(hào)：
    CSB-EP529717SXV
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母ypt7重組蛋白
  • 貨號(hào)：
    CSB-EP529717SXV-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    龐貝裂殖酵母ypt7重組蛋白
  • 貨號(hào)：
    CSB-BP529717SXV
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母ypt7重組蛋白
  • 貨號(hào)：
    CSB-MP529717SXV
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    ypt7
  • Uniprot No.：
  • 別名：
    ypt7; SPBC405.04cGTP-binding protein ypt7
  • 種屬：
    Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
  • 蛋白長(zhǎng)度：
    full length protein
  • 表達(dá)區(qū)域：
    1-205
  • 氨基酸序列
    MAGKKKHLLK VIILGESGVG KTSIMNQYVN RKFSKDYKAT IGADFLTKEV LVDDKVVTLQ LWDTAGQERF QSLGVAFYRG ADCCVLVYDV NNSKSFETLD SWRDEFLIQA SPSNPETFPF ILLGNKVDVE EQKRMVSKSK ALAFCQARGE IPYFETSAKE AINVQEAFET VAKLALENMD SDDIAADFTD PIHLDMESQK TSCYC
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Ypt/Rab-type GTPases are key regulators of membrane trafficking and intracellular vesicular transport. They act as molecular switches that convert between GTP-bound and GDP-bound states, and regulate virtually all steps of membrane traffic from the formation of the transport vesicle at the donor membrane to its fusion at the target membrane. In the GDP-bound state, Ypt proteins are predominantly cytosolic, solubilized through the interaction with a GDP dissociation inhibitor (GDI). In the GTP-bound state, the proteins are membrane bound and interact with specific effector proteins that select cargo, promote vesicle movement, or verify the correct site of fusion. Ypt7 is necessary for trafficking from the endosome to the vacuole and for homotypic vacuole fusion. Plays an important role in sporulation.
  • 基因功能參考文獻(xiàn)：
    1. Here, we report that Ypt7p plays important roles in sporulation. PMID: 16357443
    2. Data show that the two S. pombe Rab7 homologs, Ypt7 and Ypt71, act antagonistically in regulating vacuolar morphology. PMID: 19453973
  • 亞細(xì)胞定位：
    Vacuole membrane.
  • 蛋白家族：
    Small GTPase superfamily, Rab family
  • 數(shù)據(jù)庫鏈接：