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  4. Recombinant Schizosaccharomyces pombe Transcription initiation factor TFIID subunit 14 (tfg3)

Recombinant Schizosaccharomyces pombe Transcription initiation factor TFIID subunit 14 (tfg3)

  • 中文名稱：
    龐貝裂殖酵母tfg3重組蛋白
  • 貨號(hào)：
    CSB-YP529670SXV
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母tfg3重組蛋白
  • 貨號(hào)：
    CSB-EP529670SXV
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母tfg3重組蛋白
  • 貨號(hào)：
    CSB-EP529670SXV-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    龐貝裂殖酵母tfg3重組蛋白
  • 貨號(hào)：
    CSB-BP529670SXV
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母tfg3重組蛋白
  • 貨號(hào)：
    CSB-MP529670SXV
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    tfg3
  • Uniprot No.：
  • 別名：
    tfg3; taf14; SPAC22H12.02; Transcription initiation factor TFIID subunit 14; SWI/SNF chromatin-remodeling complex subunit tfg3; SWI/SNF complex subunit tfg3; TBP-associated factor 14; TBP-associated factor 30 kDa; Transcription factor G 30 kDa subunit; Transcription initiation factor TFIIF 30 kDa subunit
  • 種屬：
    Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
  • 蛋白長(zhǎng)度：
    full length protein
  • 表達(dá)區(qū)域：
    1-241
  • 氨基酸序列
    MTTVKRTVRL ITDQNVLPGG EAAVLNDQSF PVREWSIKLV CLNPQGEETD ASFVDRVTYK LHPTFQNPTR TIRKPPFQIK EQGWGEFEME IIIYYADKGG EHRFLHYLHF QQEHYHEDIE LNINATRPGL LKALTATGEV PGYSDEGEEA RKDKRKNESE VGAGKKKAKA KPVDMDKLAE GLQKLQEDDL LQVVQMVNEN KTPDMYVRND IEGGEFHIDL YTLPDNLLLL LYSFCAKRVT M
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Functions as a component of the DNA-binding general transcription factor complex TFIID, and the RNA polymerase II associated general transcription factor complex TFIIF. Binding of TFIID to a promoter (with or without TATA element) is the initial step in preinitiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, facilitation of DNA opening and initiation of transcription. TFIIF is essential for the initiation of transcription by RNA polymerase II. TFIIF functions include the recruitment of RNA polymerase II to the promoter bound DNA-TBP-TFIIB complex, decreasing the affinity of RNA polymerase II for non-specific DNA, allowing for the subsequent recruitment of TFIIE and TFIIH, and facilitating RNA polymerase II elongation. The TAF14 subunit has stimulatory activity. Component of the SWI/SNF complex, an ATP-dependent chromatin remodeling complex, required for the positive and negative regulation of gene expression of a large number of genes. It changes chromatin structure by altering DNA-histone contacts within a nucleosome, leading eventually to a change in nucleosome position, thus facilitating or repressing binding of gene-specific transcription factors. Component of the mst2 complex which is a highly specific H3 lysine 14 (H3K14) acetyltransferase that functions together with gcn5 to regulate global levels of H3K14 acetylation (H3K14ac), critical for DNA damage checkpoint activation.
  • 基因功能參考文獻(xiàn)：
    1. Tfg3 is involved in transcriptional regulation under stress conditions, particularly at high temperatures. [Tfg3] [Taf14] PMID: 15616156
  • 亞細(xì)胞定位：
    Nucleus, nucleoplasm.
  • 蛋白家族：
    TAF14 family
  • 數(shù)據(jù)庫鏈接：