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  4. Recombinant Sheep Prion-like protein doppel (PRND)

Recombinant Sheep Prion-like protein doppel (PRND)

  • 中文名稱：
    綿羊PRND重組蛋白
  • 貨號：
    CSB-YP880883SH
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    綿羊PRND重組蛋白
  • 貨號：
    CSB-EP880883SH
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    綿羊PRND重組蛋白
  • 貨號：
    CSB-EP880883SH-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    綿羊PRND重組蛋白
  • 貨號：
    CSB-BP880883SH
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    綿羊PRND重組蛋白
  • 貨號：
    CSB-MP880883SH
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    PRND; Prion-like protein doppel; PrPLP
  • 種屬：
    Ovis aries (Sheep)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區(qū)域：
    27-154
  • 氨基酸序列
    RGIK HRIKWNRKVL PSTSQVTEAH TAEIRPGAFI KQGRKLDINF GVEGNRYYEA NYWQFPDGIH YNGCSEANVT KEKFVTSCIN ATQVANQEEL SREKQDNKLY QRVLWQLIRE LCSIKHCDFW LERG
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Required for normal acrosome reaction and for normal male fertility. Can bind Cu(2+).
  • 基因功能參考文獻：
    1. the results of the present study confirm that the identification of different PRND genotypes is important for studying the sperm capacitation process and for improving sperm cryoresistance and embryo production. Furthermore, the detection of Doppel in ejaculated ovine spermatozoa, along with its low expression after cryopreservation, strongly suggests an important physiological function of this protein in male fertility. PMID: 28442049
    2. A novel 20-bp indel within sheep PRND gene was verified in 623 tested individuals representing 4 diversity sheep breeds. PMID: 28362554
    3. Preliminary data suggest that Doppel protein may enhance in vitro spermatozoa fertilizing ability. PMID: 21806689
    4. These results constitute preliminary evidence of an association between a polymorphism in the PRND gene and scrapie susceptibility PMID: 19968641
    5. Dpl is enriched in residual bodies, which are phagocytosed and destroyed by Sertoli cells after release of sperm into the lumen of the seminiferous tubule PMID: 16390495

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  • 亞細胞定位：
    Cell membrane; Lipid-anchor, GPI-anchor.
  • 蛋白家族：
    Prion family
  • 組織特異性：
    Strongly expressed in testis. Detected at low levels in lymph node, spleen and ovary.
  • 數(shù)據(jù)庫鏈接：

    KEGG: oas:443194

    UniGene: Oar.838