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  4. Recombinant Streptomyces viridochromogenes Phosphinothricin N-acetyltransferase (pat)

Recombinant Streptomyces viridochromogenes Phosphinothricin N-acetyltransferase (pat)

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  • 中文名稱：
    Recombinant Streptomyces viridochromogenes Phosphinothricin N-acetyltransferase(pat)
  • 貨號：
    CSB-EP701139FOQ
  • 規(guī)格：
    ￥2328
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    pat
  • Uniprot No.：
  • 別名：
    pat; Phosphinothricin N-acetyltransferase; PPT N-acetyltransferase; EC 2.3.1.183; Phosphinothricin-resistance protein
  • 種屬：
    Streptomyces viridochromogenes (strain DSM 40736 / JCM 4977 / BCRC 1201 / Tue 494)
  • 蛋白長度：
    Full Length
  • 來源：
    E.coli
  • 分子量：
    36.6kDa
  • 表達(dá)區(qū)域：
    1-183aa
  • 氨基酸序列
    MSPERRPVEIRPATAADMAAVCDIVNHYIETSTVNFRTEPQTPQEWIDDLERLQDRYPWLVAEVEGVVAGIAYAGPWKARNAYDWTVESTVYVSHRHQRLGLGSTLYTHLLKSMEAQGFKSVVAVIGLPNDPSVRLHEALGYTARGTLRAAGYKHGGWHDVGFWQRDFELPAPPRPVRPVTQI
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標(biāo)簽：
    N-terminal 6xHis-SUMO-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
    Note: If you have any special requirement for the glycerol content, please remark when you place the order.
    If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    3-7 business days
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產(chǎn)品描述：

    The production of this recombinant S.viridochromogenes pat protein is just like all recombinant proteins. The process involved transfecting E.coli cells with DNA vector containing the template of recombinant DNA. The E.coli cells containing the template were then cultured so that they could transcribe and translate the pat protein. N-terminal 6xHis-SUMO tag was used in the process. The purity is 90% determined by SDS-PAGE.

    PAT is a gene providing instructions for making a protein called phosphinothricin N-acetyltransferase (also known as PPT N-acetyltransferase or phosphinothricin-resistance protein). This protein, an effector of phosphinothricin tripeptide (PTT or bialaphos) resistance, has N-acetyltransferase and phosphinothricin N-acetyltransferase activity involved in response to antibiotic and herbicide. This enzyme inactivates phosphinothricin (PPT) through a transfer of an acetyl group from acetyl CoA.

  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Inactivates phosphinothricin (PPT) by transfer of an acetyl group from acetyl CoA. This enzyme is an effector of phosphinothricin tripeptide (PTT or bialaphos) resistance.
  • 蛋白家族：
    Acetyltransferase family, PAT/BAR subfamily