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  4. Recombinant Vibrio cholerae serotype O1 Cyclic di-GMP binding protein VCA0042 (VC_A0042)

Recombinant Vibrio cholerae serotype O1 Cyclic di-GMP binding protein VCA0042 (VC_A0042)

  • 中文名稱(chēng)：
    血清型O1霍亂弧菌VC_A0042重組蛋白
  • 貨號(hào)：
    CSB-YP864593VEX
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱(chēng)：
    血清型O1霍亂弧菌VC_A0042重組蛋白
  • 貨號(hào)：
    CSB-EP864593VEX
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱(chēng)：
    血清型O1霍亂弧菌VC_A0042重組蛋白
  • 貨號(hào)：
    CSB-EP864593VEX-B
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱(chēng)：
    血清型O1霍亂弧菌VC_A0042重組蛋白
  • 貨號(hào)：
    CSB-BP864593VEX
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱(chēng)：
    血清型O1霍亂弧菌VC_A0042重組蛋白
  • 貨號(hào)：
    CSB-MP864593VEX
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    VC_A0042
  • Uniprot No.：
  • 別名：
    VC_A0042; Cyclic di-GMP binding protein VCA0042
  • 種屬：
    Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
  • 蛋白長(zhǎng)度：
    full length protein
  • 表達(dá)區(qū)域：
    1-252
  • 氨基酸序列
    MNSRPAEKID NNDGQTETPR SKTVSTINST DALAMVEHSS ELTLSITTPV GTKFVCRTPF IGTHTDKFLL VEMPKISADD LQYFFQEGFW MNIRAISPRG EGALIHFRSQ LMHILQEPVP MAFLSIPNTM QVSQLRKEPR FELNLAGKVL FDEHRGDCEL RDLSRSGCRF ITPPLGKTYQ VGDLVALEIF SDLRGTKTFP PLTGKICNLQ RSLHHARYGL EFNEEGRNNA KNLLAQLKFN GTKLTLNAEK KA
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    May act as a flagellar brake, regulating swimming and swarming in a bis-(3'-5') cyclic diguanylic acid (c-di-GMP)-dependent manner. Increasing levels of c-di-GMP lead to decreased motility (Potential). Binds bis-(3'-5') cyclic diguanylic acid (c-di-GMP) with a dissociation constant of 170 nM in the presence of 10 mM KCl and with 100 nM in its absence. Binds 1 to 2 c-di-GMP per subunit. Only 1 c-di-GMP is seen in the wild-type crystal, while 2 are seen in the mutant. Depending on the concentration of K(+) stoichiometries of 1:1, 1.43:1 and 2:1 are determined by isothermal titration calorimetry.
  • 亞細(xì)胞定位：
    Bacterial flagellum basal body.
  • 蛋白家族：
    YcgR family
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: vch:VCA0042

    STRING: 243277.VCA0042