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  4. Recombinant Xenopus laevis Eukaryotic translation initiation factor 4E (eif4e)

Recombinant Xenopus laevis Eukaryotic translation initiation factor 4E (eif4e)

  • 中文名稱：
    有爪蟾蜍eif4e重組蛋白
  • 貨號：
    CSB-YP007556XBE
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    有爪蟾蜍eif4e重組蛋白
  • 貨號：
    CSB-EP007556XBE
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    有爪蟾蜍eif4e重組蛋白
  • 貨號：
    CSB-EP007556XBE-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    有爪蟾蜍eif4e重組蛋白
  • 貨號：
    CSB-BP007556XBE
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    有爪蟾蜍eif4e重組蛋白
  • 貨號：
    CSB-MP007556XBE
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    eif4eEukaryotic translation initiation factor 4E; eIF-4E; eIF4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein
  • 種屬：
    Xenopus laevis (African clawed frog)
  • 蛋白長度：
    full length protein
  • 表達區(qū)域：
    1-213
  • 氨基酸序列
    MAAVEPENTN PQSTEEEKET GQEIVSPDQY IKHPLQNRWA LWFFKNDKSK TWQANLRLIS KFDTVEDFWA LYNHIQLSSN LMSGCDYSLF KDGIEPMWED EKNKRGGRWL ITLNKQQRRN DLDRFWLETL MCLIGESFDE HSDDVCGAVV NVRAKGDKIA IWTTEFENKD AVTHIGRVYK ERLGLPAKVV IGYQSHADTA TKSGSTTKNR FVV
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. In addition to its role in translation initiation, also acts as a regulator of translation and stability in the cytoplasm. Maternal RNA in oocytes remain in a dormant state as tacc3/maskin outcompetes eif4g to bind eif4e, thereby preventing translation. During oocyte maturation this complex dissolves and eif4g binds eif4e to allow translation of maternal RNAs.
  • 基因功能參考文獻：
    1. CDK1 and calcineurin regulate Maskin association with eIF4E and translational control of cell cycle progression PMID: 17086181
    2. CPEB, partnered with several highly conserved RNA-binding partners, inhibits protein synthesis in oocytes using a novel pairing of 4E-T and eIF4E1b PMID: 17942399
  • 亞細胞定位：
    Cytoplasm.
  • 蛋白家族：
    Eukaryotic initiation factor 4E family
  • 數(shù)據(jù)庫鏈接：

    UniGene: Xl.59