6*His Monoclonal Antibody
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中文名稱:6*His鼠單克隆抗體
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貨號(hào):CSB-MA000011M0m
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規(guī)格:¥400
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圖片:
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Western Blot
Positive WB detected in: HIS fusion protein 1 at 20ng, 10ng, 5ng, 2.5ng
All lanes: HIS antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa
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Western Blot
Positive WB detected in: HIS fusion protein 2 at 20ng, 10ng, 5ng, 2.5ng
All lanes: HIS antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:CUSABIO貨號(hào):CSB-MA000011M0m 6*His單克隆抗體是針對(duì)組氨酸標(biāo)簽(6*His)研發(fā)的高特異性檢測試劑,廣泛適用于His標(biāo)簽重組蛋白的檢測與分析。該抗體采用雜交瘤技術(shù)制備,來源于小鼠免疫球蛋白G,具有高度均一性和特異性,可精準(zhǔn)識(shí)別重組蛋白中融合表達(dá)的6*His標(biāo)簽結(jié)構(gòu)。經(jīng)嚴(yán)格驗(yàn)證,該產(chǎn)品在Western Blot(WB)和酶聯(lián)免疫吸附試驗(yàn)(ELISA)中均表現(xiàn)出優(yōu)異的檢測靈敏度,支持跨物種樣本檢測,適用于大腸桿菌、哺乳動(dòng)物細(xì)胞等多種表達(dá)系統(tǒng)中重組蛋白的表達(dá)驗(yàn)證、純化監(jiān)測及定量分析。在重組蛋白純化工藝開發(fā)、蛋白互作研究、基因工程藥物研發(fā)等科研領(lǐng)域有重要應(yīng)用價(jià)值,特別適用于需要高特異性識(shí)別His標(biāo)簽的蛋白質(zhì)組學(xué)研究。本產(chǎn)品通過嚴(yán)格的質(zhì)控體系確保批次穩(wěn)定性,為His標(biāo)簽融合蛋白的檢測提供可靠解決方案。
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別名:6*His, 6*His, His Tag, 6histag, 6*His, HHHHHH, HIS, His Tag
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宿主:Mouse
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反應(yīng)種屬:N/A
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免疫原:HHHHHH(6x His) synthetic peptide conjugate to KLH
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標(biāo)記方式:Non-conjugated
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克隆類型:Monoclonal
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抗體亞型:IgG2a
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純化方式:>95%, Protein A purified
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克隆號(hào):3G5H8
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濃度:It differs from different batches. Please contact us to confirm it.
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保存緩沖液:Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 -
產(chǎn)品提供形式:Liquid
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應(yīng)用范圍:ELISA, WB
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推薦稀釋比:
Application Recommended Dilution WB 1:500-1:2000 -
Protocols:
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儲(chǔ)存條件:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期:Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途:For Research Use Only. Not for use in diagnostic or therapeutic procedures.
引用文獻(xiàn)
- cDNA cloning, prokaryotic expression, and functional analysis of squalene synthase (SQS) in Camellia vietnamensis Huang Jiani Dai,Protein Expression and Purification,2023
- Lsr2 acts as a cyclic di-GMP receptor that promotes keto-mycolic acid synthesis and biofilm formation in mycobacteria W Wang,Nature communications,2024
- Neuronal sphingosine kinase 2 subcellular localization is altered in Alzheimer's disease brain Dominguez G.et al,Acta Neuropathol Commun,2018
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