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  4. E-Tag Monoclonal Antibody

E-Tag Monoclonal Antibody

  • 中文名稱：
    E-Tag鼠單克隆抗體
  • 貨號：
    CSB-MA000151M0m
  • 規(guī)格：
    ￥400
  • 圖片：

    • Western Blot
      Positive WB detected in: E-tagged fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng
      All lanes: E-Tag antibody at 1:1000
      Secondary
      Goat polyclonal to Mouse IgG at 1/10000 dilution
      Predicted band size: 36 kDa
      Observed band size: 36 kDa
       

    • Western Blot
      Positive WB detected in: E-tagged fusion protein
      All lanes: E-Tag antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000
      Secondary
      Goat polyclonal to Mouse IgG at 1/10000 dilution
      Predicted band size: 36 kDa
      Observed band size: 36 kDa
       

    • Immunoprecipitating E-Tag in 293F transfected whole cell lysate
      Lane 1: Mouse control IgG (1µg) instead of CSB-MA000151M0m in 293F transfected whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
      Lane 2: CSB-MA000151M0m (5µg) + 293F transfected whole cell lysate (500µg)
      Lane 3: 293F transfected whole cell lysate (20µg)

       

    • ELISA E-Tag antibody in E-tagged fusion protein and Control protein (no E-tag)
      Antigen at dilution of 1µg/ml
      E-Tag antibody at (0-0.15µg)
      Goat polyclonal to Mouse IgG at 1/50000 dilution

       

    • Western Blot
      Positive WB detected in: 1-3 lanes: E‐tagged fusion protein, 4-5 lanes: Recombinant protein without E-tagged, 6 lane: BSA-E-Tag
      All lanes: E-Tag antibody at 1:1000
      Secondary
      Goat polyclonal to Mouse IgG at 1/10000 dilution
      Predicted band size: 30, 68, 55, 67-157kDa
      Observed band size: 30, 68, 55, 67-157kDa

  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    CUSABIO貨號：CSB-MA000151M0m E-Tag單克隆抗體是針對重組蛋白研究中廣泛應(yīng)用的E-Tag標簽開發(fā)的科研工具，該標簽系統(tǒng)常用于目標蛋白的追蹤、純化及互作研究。本產(chǎn)品采用高親和力小鼠源單抗制備，能特異性識別由GAPVPYPDPLEPR氨基酸序列組成的E-Tag表位，適用于各類物種來源的E-Tag標記蛋白檢測。經(jīng)嚴格驗證可在ELISA實驗中實現(xiàn)精準定量分析，在Western Blot中清晰呈現(xiàn)10-150 kDa分子量范圍內(nèi)的靶蛋白條帶，并支持免疫共沉淀（IP）技術(shù)用于蛋白相互作用研究。該抗體適用于重組蛋白表達驗證、蛋白純化過程監(jiān)控以及蛋白質(zhì)相互作用組學等科研場景，特別在哺乳動物表達系統(tǒng)、原核表達系統(tǒng)及體外翻譯系統(tǒng)的研究中展現(xiàn)優(yōu)異性能。為分子生物學、生物化學領(lǐng)域的E-Tag標記蛋白研究提供穩(wěn)定可靠的檢測支持，助力重組蛋白功能解析及信號通路機制探索。
  • 別名：
    E-Tag
  • 宿主：
    Mouse
  • 反應(yīng)種屬：
    All
  • 免疫原：
    GAPVPYPDPLEPRC synthetic peptide conjugate to KLH
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal Antibody
  • 抗體亞型：
    IgG1
  • 純化方式：
    >95%, Protein G purified
  • 克隆號：
    11H12B3
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IP
  • 推薦稀釋比：
    Application Recommended Dilution
    ELISA 1:4000-1:8000
    WB 1:1000-1:320000
    IP 1μg-5μg
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價