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  4. Flag Tag Monoclonal Antibody

Flag Tag Monoclonal Antibody

  • 中文名稱：
    Flag Tag鼠單克隆抗體
  • 貨號：
    CSB-MA000021M0m
  • 規(guī)格：
    ￥400
  • 圖片：

    • Western Blot
      Positive WB detected in: 10ng Flag Tag fusion protein Flag Tag antibody at 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 35 kDa
      Observed band size: 35 kDa

    • Western Blot
      Positive WB detected in: Flag Tag fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.625ng, 1.5625ng, 0.78ng
      All lanes: Flag Tag antibody at 1:1000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 35 kDa
      Observed band size: 35 kDa

    • Western Blot
      Positive WB detected in: Flag Tag fusion protein 1, 2, 3, 4 at 50ng
      All lanes: Flag Tag antibody at 1:1000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 59.6, 36, 35, 42 kDa
      Observed band size: 59.6, 36, 35, 42 kDa

    • Western Blot
      Positive WB detected in: Flag Tag fusion protein at 10ng
      All lanes: Company A, Company B, Company C, Company D, CSB-MA000021M0m antibody at 1:50000, 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000, 1:3200000, 1:6400000
      Secondary
      Goat polyclonal to Mouse IgG at 1/50000 dilution
      Predicted band size: 35 kDa
      Observed band size: 35 kDa

    • Western Blot
      Positive WB detected in: Flag Tag fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng, 1.5625ng, 0.78ng, 0.39ng
      All lanes: Company A, Company B, Company C, Company D, CSB-MA000021M0m antibody at 1:1000
      Secondary
      Goat polyclonal to Mouse IgG at 1/50000 dilution
      Predicted band size: 35 kDa
      Observed band size: 35 kDa

    • Western Blot
      Positive WB detected in: 5 different recombinant proteins with Flag Tag
      All lanes: Company A, Company B, Company C, Company D, CSB-MA000021M0m antibody at 1:5000
      Secondary
      Goat polyclonal to Mouse IgG at 1/50000 dilution

    • Immunoprecipitating Flag Tag in Transfected HEK-293 cell whole cell lysate
      Lane 1: Mouse control IgG instead of CSB-MA000021M0m in Transfected HEK-293 cell whole cell lysate
      Lane 2: CSB-MA000021M0m (1μl) + Transfected HEK-293 cell whole cell lysate (500μg)
      Lane 3: Transfected HEK-293 cell whole cell lysate (20μg)
      For western blotting, the blot was detected with CSB-MA000021M0m at 1:5000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000

    • Immunoprecipitating Flag Tag in 293F transfected whole cell lysate
      Lane 1: Mouse control IgG instead of CSB-MA000021M0m in Flag Tag in 293F transfected whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
      Lane 2: Company A (1μl), Company B (1μl), Company C (1μl), Company D (1μl), CSB-MA000021M0m (1μl) + Flag Tag in 293F transfected whole cell lysate (500μg)
      Lane 3: Flag Tag in 293F transfected whole cell lysate (20μg)

    • Immunofluorescence staining of 293T cells transfected with Flag tag by CSB-MA000021M0m at 1:300, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-mouse IgG(H+L). The image on the right is the 293T cells transfected without Flag tag.

  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    CUSABIO貨號：CSB-MA000021M0m Flag標(biāo)簽單克隆抗體是針對DYKDDDDK氨基酸序列開發(fā)的高特異性科研工具，該八肽標(biāo)簽因其親水特性和可定向插入蛋白N端/C端的特性，已成為重組蛋白表達驗證的核心分子標(biāo)記。本產(chǎn)品采用穩(wěn)定雜交瘤技術(shù)制備，具備跨物種廣譜反應(yīng)性，可兼容ELISA、Western Blotting、免疫熒光和免疫沉淀四大實驗平臺，特別適用于融合蛋白表達效率驗證、亞細(xì)胞定位追蹤及蛋白互作復(fù)合物捕獲等研究場景。作為FLAG標(biāo)簽檢測的關(guān)鍵試劑，該抗體在HEK293細(xì)胞重組表達體系驗證中展現(xiàn)出優(yōu)異的信噪比性能，配合抗體重鏈特異性設(shè)計有效避免輕鏈干擾，為基因編輯驗證、信號通路蛋白互作研究提供精準(zhǔn)的分子檢測解決方案。
  • 別名：
    DDK tag, DYKDDDDK, Flag, Flag Tag
  • 宿主：
    Mouse
  • 反應(yīng)種屬：
    All
  • 免疫原：
    DYKDDDDK(Flag) synthetic peptide conjugate to KLH
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    IgG2a
  • 純化方式：
    >95%, Protein G purified
  • 克隆號：
    4G5C8
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IF, IP
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:1000-1:10000
    IF 1:100-1:300
    IP 0.5ul-2ul
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價