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  4. Myc tag Monoclonal Antibody

Myc tag Monoclonal Antibody

  • 中文名稱：
    Myc tag鼠單克隆抗體
  • 貨號：
    CSB-MA000041M0m
  • 規(guī)格：
    ￥400
  • 圖片：

    • WB: Mouse anti Myc-tagged fusion protein Monoclonal antibody at 1.6μg/ml
      Lane 1: Recombinant Myc-tagged fusion protein at 50ng
      Lane 2: Recombinant Myc-tagged fusion protein at 25ng
      Lane 3: Recombinant Myc-tagged fusion protein at 12.25ng
      Lane 4: Recombinant Myc-tagged fusion protein at 6.25ng
      Lane 5: Recombinant Myc-tagged fusion protein at 3.125ng
      Lane 6: Recombinant Myc-tagged fusion protein at 1.5625ng
      Secondary
      Goat polyclonal to Mouse IgG at 1/50000 dilution
      Predicted band size: 50 kd
      Observed band size: 50 kd

    • Immunofluorescence staining of transfected HEK293 cells with CSB-MA000041M0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

    • Immunoprecipitating MYC-tag with transfected HEK293
      Lane 1: Mouse control IgG (1µg) instead of CSB-MA000041M0m in transfected HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
      Lane 2: CSB-MA000041M0m (6µg) + transfected HEK293 whole cell lysate (1mg)
      Lane 3: Transfected HEK293 whole cell lysate (10µg)

  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    CUSABIO貨號：CSB-MA000041M0m Myc標簽單克隆抗體是針對Myc表位標簽研發(fā)的高特異性科研試劑，該抗體采用雜交瘤技術(shù)制備，具有批次穩(wěn)定性和優(yōu)異的抗原結(jié)合能力。Myc標簽作為分子生物學研究中廣泛應(yīng)用的短肽標簽（EQKLISEEDL），常被用于重組蛋白表達系統(tǒng)的追蹤、純化及功能驗證。本產(chǎn)品通過嚴格驗證，適用于Western Blot、免疫熒光、免疫沉淀及ELISA等多種實驗體系，可精準識別C端或N端融合的Myc標簽蛋白。其廣譜物種兼容性覆蓋哺乳動物、昆蟲及酵母等表達系統(tǒng)，特別適用于重組蛋白表達驗證、亞細胞定位分析以及蛋白相互作用研究等實驗場景。該抗體經(jīng)多次親和純化處理，在1:1000工作濃度下展現(xiàn)出清晰的檢測信號與低背景特性，是蛋白質(zhì)組學研究和基因功能分析的關(guān)鍵工具試劑。實驗方案推薦使用含5%脫脂牛奶的TBST封閉液進行膜封閉，搭配辣根過氧化物酶標記二抗實現(xiàn)可視化檢測，助力科研人員高效完成分子互作機制、信號通路解析等前沿課題研究。
  • 別名：
    bHLHe39, c Myc, MRTL, MYC, Myc proto oncogene protein, MYC tag, Proto oncogene c Myc, Transcription factor p64
  • 宿主：
    Mouse
  • 反應(yīng)種屬：
    N/A
  • 免疫原：
    EQKLISEEDL (Myc) synthetic peptide conjugate to KLH
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    IgG1
  • 純化方式：
    >95%, Protein G purified
  • 克隆號：
    5F92F9
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IF, IP
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IF 1:100-1:300
    IP 1:1000-1:1500
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

問答及客戶評論

■ 客戶評論
  • Western blot review for CD146 Monoclonal Antibody
    Tested application: WB
    Test sample: Cells
    Sample volume: 4μg
    Electrophoresis parameters: 100V
    Primary antibody dilution ratio: 1:500
    Review: Antibody specificity is very good.
  • Western blot review for CD146 Monoclonal Antibody
    Tested application: WB
    Test sample: HEK293 cell lysate
    Sample volume: 20μg
    Electrophoresis parameters: 80V/100V
    Primary antibody dilution ratio: 1:1000
    Review: Myc antibody has good specificity.