V5-Tag Monoclonal Antibody
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中文名稱:V5-Tag鼠單克隆抗體
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貨號:CSB-MA000160
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規(guī)格:¥1090
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圖片:
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IP antibody use: 5µg V5 Mouse IgG1 per ml Lysate, WB 1: 5000
Lane 1: untransfected 293 cell lysate
Lane 2: transfected 293 cell lysate with V5-tag fusion protein
Lane 3: IP (untransfted 293 + anti-V5 mAb + Protein G agarose)
Lane 4: IP (transfected 293 + normal Mouse IgG + Protein G agarose)
Lane 5: IP (transfected 293 + anti-V5 mAb + Protein G agarose) -
IF analysis of 293 cells transfected with a V5-tag protein,1:2000 dilution (blue DAPI, red anti-V5)
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1µg V5 fusion protein + Primary antibody dilution at 1) 1:5000 2) 1:10000
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:CUSABIO貨號:CSB-MA000160 V5-Tag單克隆抗體是針對重組蛋白研究中廣泛應(yīng)用的V5表位標簽開發(fā)的科研專用試劑,該表位標簽源于副流感病毒血凝素神經(jīng)氨酸酶蛋白,因其高特異性與低交叉反應(yīng)特性成為蛋白標記及追蹤實驗的首選工具。本產(chǎn)品采用雜交瘤技術(shù)制備,經(jīng)過ELISA、免疫印跡(WB)、免疫熒光(IF)和免疫沉淀(IP)等多平臺驗證,能精準識別帶有V5標簽的重組融合蛋白。其廣譜物種適用性支持跨物種樣本分析,適用于重組蛋白表達驗證、蛋白質(zhì)相互作用網(wǎng)絡(luò)解析、亞細胞定位研究等實驗體系,尤其在多組學(xué)聯(lián)合分析中可配合His標簽或GST標簽抗體實現(xiàn)共表達蛋白的同步檢測。該抗體經(jīng)嚴格質(zhì)控確保批次穩(wěn)定性,為基因功能研究、信號通路探索及蛋白結(jié)構(gòu)解析提供高靈敏度的檢測解決方案,滿足分子生物學(xué)、細胞生物學(xué)等領(lǐng)域的科研需求。
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宿主:Mouse
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反應(yīng)種屬:N/A
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免疫原:Synthetic Peptide
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標記方式:Non-conjugated
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抗體亞型:IgG
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克隆號:18G5
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濃度:It differs from different batches. Please contact us to confirm it.
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保存緩沖液:PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
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產(chǎn)品提供形式:Liquid
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應(yīng)用范圍:ELISA, WB, IF, IP
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推薦稀釋比:
Application Recommended Dilution WB 1:500-1:5000 IF 1:50-1:200 IP 1:200-1:2000 -
Protocols:
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儲存條件:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期:Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途:For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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