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Avi-Tag Monoclonal Antibody

  • 中文名稱：
    Avi-Tag鼠單克隆抗體
  • 貨號(hào)：
    CSB-MA000101M0m
  • 規(guī)格：
    ￥400
  • 圖片：
    • Western Blot
      Positive WB detected in: 50ng Recombinant protein with Avi tag from E.coli and Mammalian cell All lanes: Avi tag antibody at 1:1000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 40, 35, 30, 70, 40, 33, 35, 40 KDa
      Observed band size: 40, 35, 30, 70, 40, 33, 35, 4 KDa
      Exposure time：1min
    • Western Blot
      Positive WB detected in: 50ng Recombinant protein with Avi tag from E.coli All lanes: Avi tag antibody at 1:2000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 81.2, 67, 66.9, 66.9 KDa
      Observed band size: 82, 67, 67, 67KDa
      Exposure time：5min
    • Western Blot
      Positive WB detected in: Recombinant protein from Mammalian cell with Avi tag from E.coli at 100ng, 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng All lanes: Avi tag antibody at 1:1000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 40 KDa
      Observed band size: 40 KDa
      Exposure time：1min
    • Western Blot
      Positive WB detected in: 50ng Recombinant protein with Avi tag from Mammalian cell Avi tag antibody at 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000, 1:640000, 1:1280000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 40 KDa
      Observed band size: 40 KDa
      Exposure time：5min
    • Immunoprecipitating Avi tag in 293F whole cell lysate transfected with avi tag
      Lane 1: Mouse control IgG instead of CSB-MA000101M0m in 293F whole cell lysate transfected with avi tag. Lane 2: CSB-MA000101M0m (1μg) + 293F whole cell lysate transfected with avi tag (500μg)
      Lane 3: 293F whole cell lysate transfected with avi tag(40μg)
      For western blotting, the blot was detected with CSB-MA000101M0m at 1:1000, and a HRP-conjugated Goat anti Mouse antibody was used as the secondary antibody at 1:50000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    CUSABIO貨號(hào)：CSB-MA000101M0m Avi-Tag單克隆抗體是針對(duì)生物素化標(biāo)簽系統(tǒng)開發(fā)的高特異性科研試劑，其靶向的AVI標(biāo)簽是廣泛應(yīng)用于重組蛋白標(biāo)記領(lǐng)域的15個(gè)氨基酸短肽序列，通過與鏈霉親和素的高親和力結(jié)合，顯著提升蛋白純化效率及檢測(cè)靈敏度。本產(chǎn)品采用雜交瘤技術(shù)制備，具備嚴(yán)格的批間一致性，經(jīng)實(shí)驗(yàn)驗(yàn)證可特異性識(shí)別AVI標(biāo)簽而不與天然生物素發(fā)生交叉反應(yīng)，適用于ELISA、Western Blot和免疫沉淀（IP）三大核心蛋白研究技術(shù)平臺(tái)。在重組蛋白表達(dá)驗(yàn)證實(shí)驗(yàn)中，該抗體能有效檢測(cè)哺乳動(dòng)物、昆蟲等多種表達(dá)系統(tǒng)產(chǎn)生的AVI標(biāo)記蛋白；在蛋白互作研究中，可通過IP實(shí)驗(yàn)高效富集標(biāo)記蛋白復(fù)合物；其Western Blot應(yīng)用可精準(zhǔn)識(shí)別1-100 kDa分子量范圍內(nèi)的標(biāo)記靶蛋白。該抗體已通過多種物種樣本驗(yàn)證，為基因編輯驗(yàn)證、蛋白互作組學(xué)研究和體外診斷試劑開發(fā)提供關(guān)鍵工具，特別適用于需要高靈敏度檢測(cè)的生物制藥研發(fā)、蛋白質(zhì)工程優(yōu)化等生命科學(xué)領(lǐng)域。本產(chǎn)品嚴(yán)格遵循科研試劑標(biāo)準(zhǔn)，配套提供詳細(xì)技術(shù)參數(shù)及實(shí)驗(yàn)方案，助力研究人員實(shí)現(xiàn)精準(zhǔn)高效的AVI標(biāo)記蛋白檢測(cè)與功能解析。
  • 產(chǎn)品名稱：
    Mouse anti-All AVI Monoclonal antibody
  • 基因名：
    AVI
  • 宿主：
    Mouse
  • 反應(yīng)種屬：
    All
  • 免疫原：
    Synthetic Peptide
  • 免疫原種屬：
    All
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    IgG2a
  • 純化方式：
    >95%, Protein G purified
  • 克隆號(hào)：
    9G3B5
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IP
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:1000-1:640000
    IP 2μl-5μl
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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