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  4. His Monoclonal Antibody

His Monoclonal Antibody

  • 中文名稱：
    His鼠單克隆抗體
  • 貨號：
    CSB-MA000011M1m
  • 規(guī)格：
    ￥400
  • 圖片：
    • Western Blot
      Positive WB detected in: 200ng recombinant protein with His tag His antibody at 1:8w, 1:16w, 1:32w, 1:64w, 1:128w, 1:256w
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 43.4 KDa
      Observed band size: 43.4 KDa
      Exposure time：5min
    • Western Blot
      Positive WB detected in: 200ng recombinant protein with His tag His antibody at 1:2w, 1:4w, 1:8w, 1:16w, 1:32w, 1:64w
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size:62.5 KDa
      Observed band size: 66 KDa
      Exposure time：5min
    • Western Blot
      Positive WB detected in:200ng of four Recombinant protein with his tag All lanes: His antibody at 1:5000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 43.4, 62.5,56,80.6 KDa
      Observed band size:43.4, 66, 57 ,90 KDa
      Exposure time：5min
    • Immunoprecipitating His in 293 whole cell lysate transfected with His
      Lane 1: control IgG instead of 2F4B5 in 293 whole cell lysate transfected with His. Lane 2: 2F4B5 antibody (2μg) + 293 whole cell lysate transfected with His (500μg)
      Lane 3: 293 whole cell lysate transfected with His (5μg)
      For western blotting, the blot was detected with 2F4B5 antibody at 1:10000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    該His Tag單克隆抗體（CUSABIO貨號：CSB-MA000011M1m）專為高效檢測重組蛋白中廣泛應(yīng)用的6×組氨酸標(biāo)簽設(shè)計，采用雜交瘤技術(shù)制備確保批次穩(wěn)定性，在Western Blot實驗中可適應(yīng)1:1000至1:640,000的寬泛稀釋范圍，免疫沉淀實驗僅需2-5μL即可有效捕獲目標(biāo)蛋白。該抗體具有優(yōu)異的種屬兼容性，經(jīng)嚴(yán)格驗證適用于ELISA、WB、IP三大核心實驗體系，特別適合重組表達(dá)蛋白的定性定量分析、蛋白相互作用研究及高通量篩選等科研場景。其高親和力特性可精準(zhǔn)識別His標(biāo)簽蛋白，在變性或天然條件下均保持穩(wěn)定結(jié)合能力，為蛋白質(zhì)純化工藝開發(fā)、表達(dá)驗證及功能研究提供可靠工具。作為His標(biāo)簽檢測領(lǐng)域的優(yōu)選試劑，該產(chǎn)品憑借靈敏的檢測限和低交叉反應(yīng)性特點，廣泛應(yīng)用于分子生物學(xué)、結(jié)構(gòu)生物學(xué)及生物制藥相關(guān)的基礎(chǔ)研究中。
  • 別名：
    6*His, 6*His, His Tag, 6histag, 6*His, HHHHHH, HIS, His Tag
  • 宿主：
    Mouse
  • 反應(yīng)種屬：
    All
  • 免疫原：
    The immunogen is a mixture of various His-tagged proteins
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    IgG1
  • 純化方式：
    >95%, Protein G purified
  • 克隆號：
    2F4B5
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IP
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:1000-1:640000
    IP 2μl-5μl
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價