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V5-Tag Monoclonal Antibody

  • 中文名稱：
    V5-Tag鼠單克隆抗體
  • 貨號：
    CSB-MA000161M0m
  • 規(guī)格：
    ￥400
  • 圖片：
    • Western Blot
      Positive WB detected in: 50ng V5-tagged fusion protein
      V5 Tag antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 37.8 KDa
      Observed band size: 43 KDa
      Exposure time: 10S
    • Western Blot
      Positive WB detected in: Mouse anti V5 tag Monoclonal antibody at 1:5000
      Lane 1: Recombinant V5-tagged fusion protein at 50ng
      Lane 2: Recombinant V5-tagged fusion protein at 25ng
      Lane 3: Recombinant V5-tagged fusion protein at 12.25ng
      Lane 4: Recombinant V5-tagged fusion protein at 6.25ng
      Secondary
      Goat polyclonal to Mouse IgG at 1/50000 dilution
      Predicted band size: 37.8 kd
      Observed band size: 43 kd
      Exposure time: 5min
    • Western Blot
      Positive WB detected in: V5-tagged fusion protein 1, 2, 3, 4 at 50ng
      All lanes: V5 antibody at 1:2000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 50.2, 47.6, 66.7, 37.8 KDa
      Observed band size: 52, 47, 67, 42 KDa
      Exposure time: 30s
    • Western Blot
      Positive WB detected in: V5-tagged fusion protein 1, 2, 3, 4, at 50ng
      All lanes: V5 antibody at 1:2000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 31.9, 20.4, 24.2, 53.4 KDa
      Observed band size: 32, 21, 28, 58 KDa
      Exposure time: 5min
    • Immunofluorescence staining of transfected HEK293F cells with CSB-MA000161M0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor R-PE Goat Anti-Mouse IgG(H+L).
    • Immunoprecipitating V5 Tag in Transfected HEK293F cells whole cell lysate
      Lane 1: Mouse control IgG1 instead of CSB-MA000161M0m in Transfected HEK293F cells whole cell lysate
      Lane 2: CSB-MA000161M0m (2.5μl) + Transfected HEK293F cells whole cell lysate (500μg)
      Lane 3: Transfected HEK293F cells whole cell lysate (20μg)
      For western blotting, the blot was detected with CSB-MA000161M0m at 1:5000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000
    • Overlay histogram showing untransfected HEK293F cells (green line) or transfected HEK293F cells (red line) stained with CSB-MA000161M0m. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1:200/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Acquisition of >10,000 events was performed.
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    V5-Tag單克隆抗體（CUSABIO貨號：CSB-MA000161M0m）是針對人工合成V5表位標(biāo)簽開發(fā)的高特異性科研工具，該標(biāo)簽廣泛應(yīng)用于重組蛋白追蹤、純化及功能驗證領(lǐng)域。本產(chǎn)品采用單克隆抗體技術(shù)制備，具有廣譜物種兼容性，可識別融合表達(dá)于目標(biāo)蛋白N端或C端的V5標(biāo)簽序列，適用于Western Blot、免疫熒光染色、免疫共沉淀及流式細(xì)胞術(shù)等多重檢測平臺，實驗數(shù)據(jù)穩(wěn)定可靠。在蛋白表達(dá)驗證、亞細(xì)胞定位分析、蛋白互作研究等場景中表現(xiàn)優(yōu)異，尤其適合需要高靈敏度檢測的哺乳動物表達(dá)系統(tǒng)研究。該抗體經(jīng)嚴(yán)格質(zhì)控驗證，批次一致性優(yōu)異，1:1000推薦稀釋比可滿足多數(shù)實驗體系需求，為基因編輯驗證、信號通路機制探索及蛋白組學(xué)研究提供高效解決方案。其多平臺兼容特性顯著提升實驗設(shè)計靈活性，是分子生物學(xué)、細(xì)胞生物學(xué)領(lǐng)域研究者的優(yōu)選試劑。
  • 基因名：
    V5-Tag
  • 宿主：
    Mouse
  • 反應(yīng)種屬：
    All
  • 免疫原：
    GKPIPNPLLGLDST synthetic peptide conjugate to KLH
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    IgG1
  • 純化方式：
    >95%, Protein G purified
  • 克隆號：
    8A7A8
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IF, IP, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:2000-1:80000
    IF 1:100-1:200
    IP 2μl-5μl
    FC 1:100-1:300
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價