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  4. Recombinant Arabidopsis thaliana Calcium-activated outward-rectifying potassium channel 1 (KCO1)

Recombinant Arabidopsis thaliana Calcium-activated outward-rectifying potassium channel 1 (KCO1)

  • 中文名稱：
    擬南芥TPK1重組蛋白
  • 貨號(hào)：
    CSB-CF814494DOA
  • 規(guī)格：
  • 來(lái)源：
    in vitro E.coli expression system
  • 其他：

產(chǎn)品詳情

  • 基因名：
    TPK1
  • Uniprot No.：
  • 別名：
    TPK1; KCO1; At5g55630; MDF20.7; Two-pore potassium channel 1; AtTPK1; Calcium-activated outward-rectifying potassium channel 1; AtKCO1
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長(zhǎng)度：
    full length protein
  • 表達(dá)區(qū)域：
    1-363
  • 氨基酸序列
    MSSDAARTPLLPTEKIDTMAQDFNLNSRTSSSRKRRLRRSRSAPRGDCMYNDDVKIDEPP PHPSKIPMFSDLNPNLRRVIMFLALYLTIGTLCFYLVRDQISGHKTSGVVDALYFCIVTM TTVGYGDLVPNSSASRLLACAFVFSGMVLVGHLLSRAADYLVEKQEALLVRAFHLRQSFG PTDILKELHTNKLRYKCYATCLVLVVLFIVGTIFLVMVEKMPVISAFYCVCSTVTTLGYG DKSFNSEAGRLFAVFWILTSSICLAQFFLYVAELNTENKQRALVKWVLTRRITNNDLEAA DLDEDGVVGAAEFIVYKLKEMGKIDEKDISGIMDEFEQLDYDESGTLTTSDIVLAQTTSQ IQR
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標(biāo)簽：
    N-terminal 10xHis-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Voltage-independent, large conductance and potassium-selective tonoplast ion channel. Regulated by cytoplasmic calcium and pH. Does not mediate slow-vacuolar (SV) ionic currents, but essential to establish VK currents. Has some permeability for Rb(+) and NH(4)(+), but none for Na(+), Cs(+) or Li(+). Involved in intracellular K(+) redistribution and/or K(+) retranslocation between different tissues.
  • 基因功能參考文獻(xiàn)：
    1. ATKCO1 can form functional potassium transport systems in E. coli PMID: 23656881
    2. TPK1 must assemble correctly to pass endoplasmic reticulum quality control. PMID: 21697507
    3. data clearly demonstrate that TPK1 (KCO1) is a voltage-independent, Ca(2+)-activated, K(+)-selective ion channel in the vacuolar membrane that does not mediate SV-type ionic currents [TPK1] PMID: 16113216
    4. The two-pore channel TPK1 gene encodes the vacuolar K+ conductance and plays a role in K+ homeostasis. PMID: 17563365
    5. findings show that AtTPK1 (KCO1), interacts with 14-3-3 proteins (general regulating factors, GRFs; TPK1 seems to provide for a Ca(2+)- and 14-3-3-sensitive mechanism capable of controlling cytoplasmic potassium homeostasis in plants PMID: 17764516

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  • 亞細(xì)胞定位：
    Vacuole membrane; Multi-pass membrane protein. Note=Tonoplast.
  • 蛋白家族：
    Two pore domain potassium channel (TC 1.A.1.7) family
  • 組織特異性：
    Detected in mesophyll cells, guard cells and vascular tissues of the leaves. Expressed in the hilum, where the funiculus is attached during fruit maturation and in the embryo. Also expressed at a lower level in seedlings, root tips and elongation zones, a
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: ath:AT5G55630

    STRING: 3702.AT5G55630.1

    UniGene: At.20254