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  4. Recombinant Arabidopsis thaliana Translocase of chloroplast 33, chloroplastic (TOC33)

Recombinant Arabidopsis thaliana Translocase of chloroplast 33, chloroplastic (TOC33)

  • 中文名稱：
    擬南芥TOC33重組蛋白
  • 貨號(hào)：
    CSB-CF521283DOA
  • 規(guī)格：
  • 來(lái)源：
    in vitro E.coli expression system
  • 其他：

產(chǎn)品詳情

  • 基因名：
    TOC33
  • Uniprot No.：
  • 別名：
    TOC33; PPI1; At1g02280; T7I23.11; Translocase of chloroplast 33, chloroplastic; AtToc33; 33 kDa chloroplast outer envelope protein; Plastid protein import 1
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長(zhǎng)度：
    full length protein
  • 表達(dá)區(qū)域：
    1-297
  • 氨基酸序列
    MGSLVREWVGFQQFPAATQEKLIEFFGKLKQKDMNSMTVLVLGKGGVGKSSTVNSLIGEQ VVRVSPFQAEGLRPVMVSRTMGGFTINIIDTPGLVEAGYVNHQALELIKGFLVNRTIDVL LYVDRLDVYRVDELDKQVVIAITQTFGKEIWCKTLLVLTHAQFSPPDELSYETFSSKRSD SLLKTIRAGSKMRKQEFEDSAIAVVYAENSGRCSKNDKDEKALPNGEAWIPNLVKAITDV ATNQRKAIHVDKKMVDGSYSDDKGKKLIPLIIGAQYLIVKMIQGAIRNDIKTSGKPL
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標(biāo)簽：
    N-terminal 10xHis-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    GTPase involved in protein precursor import into chloroplasts. Seems to recognize chloroplast-destined precursor proteins and regulate their presentation to the translocation channel through GTP hydrolysis. Binds GTP, GDP, XTP, but not ATP. Probably specialized in the import of nuclear encoded photosynthetic preproteins from the cytoplasm to the chloroplast, especially during early development stages.
  • 基因功能參考文獻(xiàn)：
    1. [review] Homodimerization of Toc33 prevents nucleotide exchange, thereby locking the receptor in the guanosine diphosphate (GDP)-loaded state and preventing further activity. PMID: 21568939
    2. Pre-protein binding induces a dissociation of the Toc33 homodimer and results in exchange of guanosine diphosphate (GDP) for guanosine triphosphate (GTP). PMID: 21434866
    3. Neither nucleotide binding nor dimerization at atToc33 is essential for chloroplast import. PMID: 20444229
    4. The role of Toc33 protein in biological transport of proteins in the protochlorophyllide-dependent pathway in A. thaliana. PMID: 15773849
    5. The data suggest that atToc33 phosphorylation may not play an important role in vivo. PMID: 16412428
    6. Chloroplast biogenesis and photosynthetic activity are impaired in the mutants during the early developmental stage, which is consistent with the requirement of atToc33 in young photosynthetic tissues. PMID: 18054337
    7. Dimerization is independent of nucleotide loading state, based on crystal structures of dimeric Pisum sativum Toc34 and monomeric Arabidopsis thaliana Toc33. PMID: 18400179
    8. Toc33 GTPase homodimerization and protein translocation into chloroplasts are pH sensitive. PMID: 19001421

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  • 亞細(xì)胞定位：
    Plastid, chloroplast outer membrane; Single-pass membrane protein. Note=May contain beta barrel transmembrane regions.
  • 蛋白家族：
    TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily, AIG1/Toc34/Toc159-like paraseptin GTPase family, TOC34 subfamily
  • 組織特異性：
    Mostly expressed in seedlings and flowers, and, to a lower extent, in roots, stems, and leaves.
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: ath:AT1G02280

    STRING: 3702.AT1G02280.1

    UniGene: At.10710