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  4. Recombinant Danio rerio Rhodopsin (rho)

Recombinant Danio rerio Rhodopsin (rho)

  • 中文名稱：
    斑馬魚rho重組蛋白
  • 貨號：
    CSB-CF019681DIL
  • 規(guī)格：
  • 來源：
    in vitro E.coli expression system
  • 其他：

產(chǎn)品詳情

  • 基因名：
  • Uniprot No.：
  • 別名：
    rho; zfo2; Rhodopsin
  • 種屬：
    Danio rerio (Zebrafish) (Brachydanio rerio)
  • 蛋白長度：
    full length protein
  • 表達(dá)區(qū)域：
    1-354
  • 氨基酸序列
    MNGTEGPAFYVPMSNATGVVRSPYEYPQYYLVAPWAYGLLAAYMFFLIITGFPVNFLTLY VTIEHKKLRTPLNYILLNLAIADLFMVFGGFTTTMYTSLHGYFVFGRLGCNLEGFFATLG GEMGLWSLVVLAIERWMVVCKPVSNFRFGENHAIMGVAFTWVMACSCAVPPLVGWSRYIP EGMQCSCGVDYYTRTPGVNNESFVIYMFIVHFFIPLIVIFFCYGRLVCTVKEAAAQQQES ETTQRAEREVTRMVIIMVIAFLICWLPYAGVAWYIFTHQGSEFGPVFMTLPAFFAKTSAV YNPCIYICMNKQFRHCMITTLCCGKNPFEEEEGASTTASKTEASSVSSSSVSPA
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標(biāo)簽：
    N-terminal 10xHis-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Photoreceptor required for image-forming vision at low light intensity. While most salt water fish species use retinal as chromophore, most freshwater fish use 3-dehydroretinal, or a mixture of retinal and 3-dehydroretinal. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change that activates signaling via G-proteins. Subsequent receptor phosphorylation mediates displacement of the bound G-protein alpha subunit by arrestin and terminates signaling.
  • 基因功能參考文獻(xiàn)：
    1. The efficiency of CRISPR/Cas9 for gene targeting, coupled with the large number of mutations associated with retinitis pigmentosa, provided a backdrop for the rapid isolation of novel alleles in zebrafish that phenocopy disease. PMID: 30210230
    2. overexpression of full-length rhodopsin or its cytoplasmic tail domain, but not of rhodopsin lacking the cytoplasmic tail, exacerbated rod degeneration in kif3a mutants, implying an important role of the cytoplasmic tail in rod degeneration. PMID: 28855254
    3. expression as well as the protein localization of rhodopsin in the zebrafish from larval to adult stage were demonstrated; results demonstrated the involvement of rhodopsin in the zebrafish pineal gland physiology particularly in light capture during the zebrafish lifespan PMID: 22306032
    4. Mitogen-associated protein kinase and protein kinase A regulate rhodopsin transcription through parallel signal transduction pathways PMID: 17183589
  • 亞細(xì)胞定位：
    Membrane; Multi-pass membrane protein. Cell projection, cilium, photoreceptor outer segment.
  • 蛋白家族：
    G-protein coupled receptor 1 family, Opsin subfamily
  • 組織特異性：
    Retinal rod photoreceptor cells, predominantly in the outer segments (at protein level). Retinal rod photoreceptor cells.
  • 數(shù)據(jù)庫鏈接：

    KEGG: dre:30295

    STRING: 7955.ENSDARP00000011562

    UniGene: Dr.354