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  4. Recombinant Drosophila melanogaster Gustatory receptor 5a for trehalose (Gr5a)

Recombinant Drosophila melanogaster Gustatory receptor 5a for trehalose (Gr5a)

  • 中文名稱：
    黑腹果蠅Gr5a重組蛋白
  • 貨號：
    CSB-CF896191DLU
  • 規(guī)格：
  • 來源：
    in vitro E.coli expression system
  • 其他：

產品詳情

  • 基因名：
    Gr5a
  • Uniprot No.：
  • 別名：
    Gr5a; GRLU.7; Tre; CG15779; Gustatory receptor 5a for trehalose; Trehalose receptor
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    full length protein
  • 表達區(qū)域：
    1-444
  • 氨基酸序列
    MRQLKGRNRCNRAVRHLKIQGKMWLKNLKSGLEQIRESQVRGTRKNFLHDGSFHEAVAPV LAVAQCFCLMPVCGISAPTYRGLSFNRRSWRFWYSSLYLCSTSVDLAFSIRRVAHSVLDV RSVEPIVFHVSILIASWQFLNLAQLWPGLMRHWAAVERRLPGYTCCLQRARPARRLKLVA FVLLVVSLMEHLLSIISVVYYDFCPRRSDPVESYLLGASAQLFEVFPYSNWLAWLGKIQN VLLTFGWSYMDIFLMMLGMGLSEMLARLNRSLEQQVRQPMPEAYWTWSRTLYRSIVELIR EVDDAVSGIMLISFGSNLYFICLQLLKSINTMPSSAHAVYFYFSLLFLLSRSTAVLLFVS AINDQAREPLRLLRLVPLKGYHPEVFRFAAELASDQVALTGLKFFNVTRKLFLAMAGTVA TYELVLIQFHEDKKTWDCSPFNLD
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標簽：
    N-terminal 10xHis-tagged
  • 產品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Gustatory receptor required for response to the sugar trehalose in taste neurons. Gr5a neurons selectively respond to sugars, in contrast to Gr66a cells which respond to bitter compounds. Flies are attracted to sugars and avoid bitter substances, suggesting that Gr5a neuron activity is sufficient to mediate acceptance behavior. Sugar signal transduction occurs through coupling with G-proteins such as Galpha49B and G-salpha60A.
  • 基因功能參考文獻：
    1. The detection of sugar via Gr5a gustatory receptor neurons is necessary for food-promoted male aggression. PMID: 25162609
    2. necessary for normal trehalose detection PMID: 19174988
    3. expressed in neurons of taste sensilla located on the labellum and legs PMID: 14523229
    4. Causes of the observed pattern of nucleotide variation in the gustatory receptor Gr5a gene. PMID: 15342513
    5. analysis of trehalose sensitivity of the gustatory receptor neurons expressing wild-type, mutant and ectopic Gr5a in Drosophila PMID: 15738154
    6. single P-element insertions in the intergenic region between Gr5a and Tre1 exert complex pleiotropic effects on fitness traits, including selective nutrient intake, life span, and resistance to starvation and heat stress PMID: 16783380
    7. Gustator receptors would be candidate sugar receptors because Gr5a-expressing cells function in the responses to attractive tastants. PMID: 17715294
    8. The simplest interpretation of our results is that Gr5a and Gr64a are each capable of functioning independently of each other within individual sugar neurons and that they are the primary receptors used in the labellum to detect sugars. PMID: 17988633

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  • 亞細胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Insect chemoreceptor superfamily, Gustatory receptor (GR) family, Gr5a subfamily
  • 組織特異性：
    Expressed in labellar chemosensory neurons.
  • 數據庫鏈接：