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  4. Recombinant Lactococcus lactis subsp. lactis Multidrug resistance ABC transporter ATP-binding and permease protein (lmrA)

Recombinant Lactococcus lactis subsp. lactis Multidrug resistance ABC transporter ATP-binding and permease protein (lmrA)

  • 中文名稱(chēng)：
    乳酸乳球菌亞屬。乳酸lmrA重組蛋白
  • 貨號(hào)：
    CSB-CF880302LNG
  • 規(guī)格：
  • 來(lái)源：
    in vitro E.coli expression system
  • 其他：

產(chǎn)品詳情

  • 基因名：
    lmrA
  • Uniprot No.：
  • 別名：
    lmrA; LL0711; L116532; Multidrug resistance ABC transporter ATP-binding and permease protein
  • 種屬：
    Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
  • 蛋白長(zhǎng)度：
    full length protein
  • 表達(dá)區(qū)域：
    1-590
  • 氨基酸序列
    MERGPQMANRIEGKAVDKTSIKHFIKLIRAAKPRYLFFIIGILAGIVGTLIQLQVPKMVQ PLVNSFGHGVNGGKVALVIALYIGSAAVSAIAAIVLGIFGESVVKNLRTRVWDKMIHLPV KYFDEVKTGEMSSRLANDTTQVKNLIANSIPQAFTSILLLVGSIVFMLQMQWRLTLAMII AVPVVMLIMFPIMTFGQKIGRTRQDSLANFQGIASESLSEIRLVKSSNAEKQASKKAEND VNALYKIGVKEAIFDGLMSPVMMLSMMLMIFGLLAYGIYLISTGVMSLGTLLGMMMYLMN LIGAVPTVATFFTELAKASGSTGRLTELLDEEQEVLHQGESLDLEGKTLSARHVDFAYDD SEQILRDISFEAQPNSIIAFAGPSGGGKSTIFSLLERFYQPTAGEITIDGQPIDNISLEN WRSQIGFVSQDSAIMAGTIRENLTYGLEGDYTDEDLWQVLDLAFARSFVENMPDQLNTEV GERGVKISGGQRQRLAIARAFLRNPKILMLDEATASLDSESESMVQKALDSLMKGRTTLV IAHRLSTIVDADKIYFIEKGQITGSGKHNELVATHPLYAKYVSEQLTVGQ
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標(biāo)簽：
    N-terminal 10xHis-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Efflux transporter for a variety of amphiphilic cationic compounds, including antibiotics.
  • 基因功能參考文獻(xiàn)：
    1. analysis of the effect of detergents on the activity of the ABC transporter LmrA PMID: 21651889
    2. a carboxylate has a critical role in proton conduction by the ATP-binding cassette multidrug transporter LmrA PMID: 16040836
    3. LmrA-mediated Hoechst 33342 transport in membrane vesicles is influenced by the transmembrane pH gradient due to a pH-dependent partitioning of Hoechst 33342 into the membrane. PMID: 16363806
    4. Remodelling of LmrA using the atomic coordinates of the MsbA homologue Sav1866 from Staphylococcus aureus is reported. PMID: 17624317
    5. The membrane domain of LmrA mediates an ethidium/proton co-transport reaction, suggesting a functional similarity in the mechanism of secondary-active and ABC transporters. PMID: 14685244
    6. LmrA mediates the reverse uptake of ethidium in ATP-depleted cells in the presence of an inwardly directed ethidium concentration gradient and LmrA-mediated uptake of ethidium is coupled to the catalytic cycle in the direction of ATP synthesis PMID: 14660649

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  • 亞細(xì)胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    ABC transporter superfamily, Multidrug exporter LmrA (TC 3.A.1.117.1) family
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: lla:L116532

    STRING: 272623.L116532