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Plate set up

日期:2011-03-16 11:01:57

Plate set up

If the user do this assay for the first time, it is recommended to do a pre-test with two strips. A suggested plate format for pre-test is shown below. This plate format is just available for cell supernatant, tissue homogenate,urine,or serum/plasm where the recommended dilution is not provided in the manual. The user may vary the location and type of wells present as necessary for each particular experiment.

Std0

Smpl 1(1:1)

Std1

Smpl 1(1:10)

Std2

Smpl 1(1:100)

Std3

Smpl 1(1:1000)

Std4

Smpl 2(1:1)

Std5

Smpl 2(1:10)

Std6

Smpl 2(1:100)

Std7

Smpl 2(1:1000)

 

 

Std---Standard

Smpl---Sample

(1:10)---10 fold dilution

(1:100)---100 fold dilution

(1:1000)---1000 fold dilution

 

 

 

After the pre-test, choose a suitable dilution to go on the assay. The plate format is shown below.

Std0
Std0
Smpl1
Smpl1
Smpl9
Smpl9
Smpl17
Smpl17
Smpl25
Smpl25
Std1
Std1
Smpl2
Smpl2
Smpl10
Smpl10
Smpl18
Smpl18
Smpl26
Smpl26
Std2
Std2
Smpl3
Smpl3
Smpl11
Smpl11
Smpl19
Smpl19
Smpl27
Smpl27
Std3
Std3
Smpl4
Smpl4
Smpl12
Smpl12
Smpl20
Smpl20
Smpl28
Smpl28
Std4
Std4
Smpl5
Smpl5
Smpl13
Smpl13
Smpl21
Smpl21
Smpl29
Smpl29
Std5
Std5
Smpl6
Smpl6
Smpl14
Smpl14
Smpl22
Smpl22
Smpl30
Smpl30
Std6
Std6
Smpl7
Smpl7
Smpl15
Smpl15
Smpl23
Smpl23
Smpl31
Smpl31
Std7
Std7
Smpl8
Smpl8
Smpl16
Smpl16
Smpl24
Smpl24
Smpl32
Smpl32

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