Plate set up
日期:2011-03-16 11:01:57
Plate set up
If the user do this assay for the first time, it is recommended to do a pre-test with two strips. A suggested plate format for pre-test is shown below. This plate format is just available for cell supernatant, tissue homogenate,urine,or serum/plasm where the recommended dilution is not provided in the manual. The user may vary the location and type of wells present as necessary for each particular experiment.
Std0 |
Smpl 1(1:1) |
Std1 |
Smpl 1(1:10) |
Std2 |
Smpl 1(1:100) |
Std3 |
Smpl 1(1:1000) |
Std4 |
Smpl 2(1:1) |
Std5 |
Smpl 2(1:10) |
Std6 |
Smpl 2(1:100) |
Std7 |
Smpl 2(1:1000) |
Std---Standard
Smpl---Sample
(1:10)---10 fold dilution
(1:100)---100 fold dilution
(1:1000)---1000 fold dilution
After the pre-test, choose a suitable dilution to go on the assay. The plate format is shown below.