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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Proteus mirabilis Peptide chain release factor 3 (prfC), partial CSB-YP459558EYZ
CSB-EP459558EYZ
CSB-BP459558EYZ
CSB-MP459558EYZ
CSB-EP459558EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Tryptophan synthase alpha chain (trpA) CSB-YP459559EYZ
CSB-EP459559EYZ
CSB-BP459559EYZ
CSB-MP459559EYZ
CSB-EP459559EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Ribosomal RNA small subunit methyltransferase C (rsmC) CSB-YP459560EYZ
CSB-EP459560EYZ
CSB-BP459560EYZ
CSB-MP459560EYZ
CSB-EP459560EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis 3-dehydroquinate dehydratase (aroQ) CSB-YP459561EYZ
CSB-EP459561EYZ
CSB-BP459561EYZ
CSB-MP459561EYZ
CSB-EP459561EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis 30S ribosomal protein S9 (rpsI) CSB-YP459562EYZ
CSB-EP459562EYZ
CSB-BP459562EYZ
CSB-MP459562EYZ
CSB-EP459562EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Crotonobetainyl-CoA:carnitine CoA-transferase (caiB) CSB-YP459563EYZ
CSB-EP459563EYZ
CSB-BP459563EYZ
CSB-MP459563EYZ
CSB-EP459563EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis UPF0756 membrane protein PMI1560 (PMI1560), partial CSB-YP459564EYZ1
CSB-EP459564EYZ1
CSB-BP459564EYZ1
CSB-MP459564EYZ1
CSB-EP459564EYZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Flagellar transcriptional regulator FlhD (flhD) CSB-YP459565EYZ
CSB-EP459565EYZ
CSB-BP459565EYZ
CSB-MP459565EYZ
CSB-EP459565EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Endonuclease V (nfi) CSB-YP459566EYZ
CSB-EP459566EYZ
CSB-BP459566EYZ
CSB-MP459566EYZ
CSB-EP459566EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Sulfurtransferase TusD (tusD) CSB-YP459567EYZ
CSB-EP459567EYZ
CSB-BP459567EYZ
CSB-MP459567EYZ
CSB-EP459567EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis 3-demethylubiquinone-9 3-methyltransferase (ubiG) CSB-YP459568EYZ
CSB-EP459568EYZ
CSB-BP459568EYZ
CSB-MP459568EYZ
CSB-EP459568EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Glycine--tRNA ligase beta subunit (glyS) CSB-YP459569EYZ
CSB-EP459569EYZ
CSB-BP459569EYZ
CSB-MP459569EYZ
CSB-EP459569EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Acetate kinase (ackA) CSB-YP459570EYZ
CSB-EP459570EYZ
CSB-BP459570EYZ
CSB-MP459570EYZ
CSB-EP459570EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis UPF0115 protein PMI1805 (PMI1805) CSB-YP459571EYZ
CSB-EP459571EYZ
CSB-BP459571EYZ
CSB-MP459571EYZ
CSB-EP459571EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Pimelyl-[acyl-carrier protein] methyl ester esterase (bioH) CSB-YP459572EYZ
CSB-EP459572EYZ
CSB-BP459572EYZ
CSB-MP459572EYZ
CSB-EP459572EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Chaperone protein hscA (hscA), partial CSB-YP459573EYZ
CSB-EP459573EYZ
CSB-BP459573EYZ
CSB-MP459573EYZ
CSB-EP459573EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Probable malate:quinone oxidoreductase (mqo) CSB-YP459574EYZ
CSB-EP459574EYZ
CSB-BP459574EYZ
CSB-MP459574EYZ
CSB-EP459574EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP459575EYZ
CSB-EP459575EYZ
CSB-BP459575EYZ
CSB-MP459575EYZ
CSB-EP459575EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis 50S ribosomal protein L28 (rpmB) CSB-YP459576EYZ
CSB-EP459576EYZ
CSB-BP459576EYZ
CSB-MP459576EYZ
CSB-EP459576EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis DNA gyrase inhibitor YacG (yacG) CSB-YP459577EYZ
CSB-EP459577EYZ
CSB-BP459577EYZ
CSB-MP459577EYZ
CSB-EP459577EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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