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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Mycobacterium marinum 50S ribosomal protein L36 (rpmJ) CSB-YP460118MVS
CSB-EP460118MVS
CSB-BP460118MVS
CSB-MP460118MVS
CSB-EP460118MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum 10 kDa chaperonin (groS) CSB-YP460119MVS
CSB-EP460119MVS
CSB-BP460119MVS
CSB-MP460119MVS
CSB-EP460119MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Peptidyl-tRNA hydrolase (pth) CSB-YP460120MVS
CSB-EP460120MVS
CSB-BP460120MVS
CSB-MP460120MVS
CSB-EP460120MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Homoserine O-acetyltransferase (metX) CSB-YP460121MVS
CSB-EP460121MVS
CSB-BP460121MVS
CSB-MP460121MVS
CSB-EP460121MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum 50S ribosomal protein L32 (rpmF) CSB-YP460122MVS
CSB-EP460122MVS
CSB-BP460122MVS
CSB-MP460122MVS
CSB-EP460122MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum 2-phospho-L-lactate transferase (cofD) CSB-YP460123MVS
CSB-EP460123MVS
CSB-BP460123MVS
CSB-MP460123MVS
CSB-EP460123MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Proteasome subunit alpha (prcA) CSB-YP460124MVS
CSB-EP460124MVS
CSB-BP460124MVS
CSB-MP460124MVS
CSB-EP460124MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Dihydroorotate dehydrogenase (quinone) CSB-YP460125MVS
CSB-EP460125MVS
CSB-BP460125MVS
CSB-MP460125MVS
CSB-EP460125MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum tRNA dimethylallyltransferase 1 (miaA1) CSB-YP460126MVS
CSB-EP460126MVS
CSB-BP460126MVS
CSB-MP460126MVS
CSB-EP460126MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP460127MVS
CSB-EP460127MVS
CSB-BP460127MVS
CSB-MP460127MVS
CSB-EP460127MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT) CSB-YP460128MVS
CSB-EP460128MVS
CSB-BP460128MVS
CSB-MP460128MVS
CSB-EP460128MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Putative S-adenosyl-L-methionine-dependent methyltransferase MMAR_1595 (MMAR_1595) CSB-YP460129MVS
CSB-EP460129MVS
CSB-BP460129MVS
CSB-MP460129MVS
CSB-EP460129MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Trehalose-phosphate synthase (otsA) CSB-YP460130MVS
CSB-EP460130MVS
CSB-BP460130MVS
CSB-MP460130MVS
CSB-EP460130MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP460131MVS
CSB-EP460131MVS
CSB-BP460131MVS
CSB-MP460131MVS
CSB-EP460131MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Polyphosphate kinase (ppk), partial CSB-YP460132MVS
CSB-EP460132MVS
CSB-BP460132MVS
CSB-MP460132MVS
CSB-EP460132MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Long-chain-fatty-acid--AMP ligase FadD28 (fadD28), partial CSB-YP460133MVS
CSB-EP460133MVS
CSB-BP460133MVS
CSB-MP460133MVS
CSB-EP460133MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum 50S ribosomal protein L33 1 (rpmG1) CSB-YP460134MVS
CSB-EP460134MVS
CSB-BP460134MVS
CSB-MP460134MVS
CSB-EP460134MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP460135MVS
CSB-EP460135MVS
CSB-BP460135MVS
CSB-MP460135MVS
CSB-EP460135MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum DNA repair protein recO (recO) CSB-YP460136MVS
CSB-EP460136MVS
CSB-BP460136MVS
CSB-MP460136MVS
CSB-EP460136MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum LexA repressor (lexA) CSB-YP460137MVS
CSB-EP460137MVS
CSB-BP460137MVS
CSB-MP460137MVS
CSB-EP460137MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據他們開發(fā)重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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