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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Xylella fastidiosa ATP synthase gamma chain (atpG) CSB-YP462139XBN
CSB-EP462139XBN
CSB-BP462139XBN
CSB-MP462139XBN
CSB-EP462139XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa RNA pyrophosphohydrolase (rppH) CSB-YP462140XBN
CSB-EP462140XBN
CSB-BP462140XBN
CSB-MP462140XBN
CSB-EP462140XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 50S ribosomal protein L23 (rplW) CSB-YP462141XBN
CSB-EP462141XBN
CSB-BP462141XBN
CSB-MP462141XBN
CSB-EP462141XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Lipase chaperone (lifO), partial CSB-YP462142XBN1
CSB-EP462142XBN1
CSB-BP462142XBN1
CSB-MP462142XBN1
CSB-EP462142XBN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP462143XBN
CSB-EP462143XBN
CSB-BP462143XBN
CSB-MP462143XBN
CSB-EP462143XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Cell division topological specificity factor (minE) CSB-YP462144XBN
CSB-EP462144XBN
CSB-BP462144XBN
CSB-MP462144XBN
CSB-EP462144XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP462145XBN
CSB-EP462145XBN
CSB-BP462145XBN
CSB-MP462145XBN
CSB-EP462145XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 8-amino-7-oxononanoate synthase (bioF) CSB-YP462146XBN
CSB-EP462146XBN
CSB-BP462146XBN
CSB-MP462146XBN
CSB-EP462146XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa UPF0042 nucleotide-binding protein XfasM23_0667 (XfasM23_0667) CSB-YP462147XBN
CSB-EP462147XBN
CSB-BP462147XBN
CSB-MP462147XBN
CSB-EP462147XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Threonine--tRNA ligase (thrS), partial CSB-YP462148XBN
CSB-EP462148XBN
CSB-BP462148XBN
CSB-MP462148XBN
CSB-EP462148XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 30S ribosomal protein S6 (rpsF) CSB-YP462149XBN
CSB-EP462149XBN
CSB-BP462149XBN
CSB-MP462149XBN
CSB-EP462149XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa UPF0176 protein XfasM23_2090 (XfasM23_2090) CSB-YP462150XBN
CSB-EP462150XBN
CSB-BP462150XBN
CSB-MP462150XBN
CSB-EP462150XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Protein CrcB homolog (crcB), partial CSB-YP462151XBN1
CSB-EP462151XBN1
CSB-BP462151XBN1
CSB-MP462151XBN1
CSB-EP462151XBN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Sulfite reductase [NADPH] hemoprotein beta-component (cysI), partial CSB-YP462152XBN
CSB-EP462152XBN
CSB-BP462152XBN
CSB-MP462152XBN
CSB-EP462152XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 50S ribosomal protein L7/L12 (rplL) CSB-YP462153XBN
CSB-EP462153XBN
CSB-BP462153XBN
CSB-MP462153XBN
CSB-EP462153XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 50S ribosomal protein L25 (rplY) CSB-YP462154XBN
CSB-EP462154XBN
CSB-BP462154XBN
CSB-MP462154XBN
CSB-EP462154XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 50S ribosomal protein L31 type B (rpmE2) CSB-YP462155XBN
CSB-EP462155XBN
CSB-BP462155XBN
CSB-MP462155XBN
CSB-EP462155XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa GTP cyclohydrolase folE2 (folE2) CSB-YP462156XBN
CSB-EP462156XBN
CSB-BP462156XBN
CSB-MP462156XBN
CSB-EP462156XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica Tyrosine--tRNA ligase (tyrS) CSB-YP462157BSP
CSB-EP462157BSP
CSB-BP462157BSP
CSB-MP462157BSP
CSB-EP462157BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica 50S ribosomal protein L33 (rpmG) CSB-YP462158BSP
CSB-EP462158BSP
CSB-BP462158BSP
CSB-MP462158BSP
CSB-EP462158BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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