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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Burkholderia phytofirmans Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP462559BXU
CSB-EP462559BXU
CSB-BP462559BXU
CSB-MP462559BXU
CSB-EP462559BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans 3-octaprenyl-4-hydroxybenzoate carboxy-lyase (ubiD), partial CSB-YP462560BXU
CSB-EP462560BXU
CSB-BP462560BXU
CSB-MP462560BXU
CSB-EP462560BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP462561BXU
CSB-EP462561BXU
CSB-BP462561BXU
CSB-MP462561BXU
CSB-EP462561BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans 50S ribosomal protein L17 (rplQ) CSB-YP462562BXU
CSB-EP462562BXU
CSB-BP462562BXU
CSB-MP462562BXU
CSB-EP462562BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans 50S ribosomal protein L30 (rpmD) CSB-YP462563BXU
CSB-EP462563BXU
CSB-BP462563BXU
CSB-MP462563BXU
CSB-EP462563BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans 50S ribosomal protein L5 (rplE) CSB-YP462564BXU
CSB-EP462564BXU
CSB-BP462564BXU
CSB-MP462564BXU
CSB-EP462564BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans 30S ribosomal protein S3 (rpsC) CSB-YP462565BXU
CSB-EP462565BXU
CSB-BP462565BXU
CSB-MP462565BXU
CSB-EP462565BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans 30S ribosomal protein S7 (rpsG) CSB-YP462566BXU
CSB-EP462566BXU
CSB-BP462566BXU
CSB-MP462566BXU
CSB-EP462566BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans 50S ribosomal protein L11 (rplK) CSB-YP462567BXU
CSB-EP462567BXU
CSB-BP462567BXU
CSB-MP462567BXU
CSB-EP462567BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC (mnmC), partial CSB-YP462568BXU
CSB-EP462568BXU
CSB-BP462568BXU
CSB-MP462568BXU
CSB-EP462568BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Homoserine kinase (thrB) CSB-YP462569BXU
CSB-EP462569BXU
CSB-BP462569BXU
CSB-MP462569BXU
CSB-EP462569BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Betaine aldehyde dehydrogenase (betB) CSB-YP462570BXU
CSB-EP462570BXU
CSB-BP462570BXU
CSB-MP462570BXU
CSB-EP462570BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans UPF0060 membrane protein Bphyt_4776 (Bphyt_4776), partial CSB-YP462571BXU1
CSB-EP462571BXU1
CSB-BP462571BXU1
CSB-MP462571BXU1
CSB-EP462571BXU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum ATP-dependent helicase/deoxyribonuclease subunit B (addB), partial CSB-YP462572DUG
CSB-EP462572DUG
CSB-BP462572DUG
CSB-MP462572DUG
CSB-EP462572DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP462573DUG
CSB-EP462573DUG
CSB-BP462573DUG
CSB-MP462573DUG
CSB-EP462573DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum UPF0473 protein CLL_A1177 (CLL_A1177) CSB-YP462574DUG
CSB-EP462574DUG
CSB-BP462574DUG
CSB-MP462574DUG
CSB-EP462574DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum UPF0296 protein CLL_A1210 (CLL_A1210) CSB-YP462575DUG
CSB-EP462575DUG
CSB-BP462575DUG
CSB-MP462575DUG
CSB-EP462575DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Glutamate racemase (murI) CSB-YP462576DUG
CSB-EP462576DUG
CSB-BP462576DUG
CSB-MP462576DUG
CSB-EP462576DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP462577DUG
CSB-EP462577DUG
CSB-BP462577DUG
CSB-MP462577DUG
CSB-EP462577DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Proline--tRNA ligase (proS), partial CSB-YP462578DUG
CSB-EP462578DUG
CSB-BP462578DUG
CSB-MP462578DUG
CSB-EP462578DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據他們開發(fā)重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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