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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Xanthomonas oryzae pv. oryzae Leucine--tRNA ligase (leuS), partial CSB-YP464479XBC
CSB-EP464479XBC
CSB-BP464479XBC
CSB-MP464479XBC
CSB-EP464479XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Thiazole synthase (thiG) CSB-YP464480XBC
CSB-EP464480XBC
CSB-BP464480XBC
CSB-MP464480XBC
CSB-EP464480XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP464481BXU
CSB-EP464481BXU
CSB-BP464481BXU
CSB-MP464481BXU
CSB-EP464481BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Peptidyl-tRNA hydrolase (pth) CSB-YP464482BXU
CSB-EP464482BXU
CSB-BP464482BXU
CSB-MP464482BXU
CSB-EP464482BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Glutamate--tRNA ligase (gltX) CSB-YP464483BXU
CSB-EP464483BXU
CSB-BP464483BXU
CSB-MP464483BXU
CSB-EP464483BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans GTPase Der (der) CSB-YP464484BXU
CSB-EP464484BXU
CSB-BP464484BXU
CSB-MP464484BXU
CSB-EP464484BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans 30S ribosomal protein S20 (rpsT) CSB-YP464485BXU
CSB-EP464485BXU
CSB-BP464485BXU
CSB-MP464485BXU
CSB-EP464485BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Kynurenine formamidase (kynB) CSB-YP464486BXU
CSB-EP464486BXU
CSB-BP464486BXU
CSB-MP464486BXU
CSB-EP464486BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP464487BXU
CSB-EP464487BXU
CSB-BP464487BXU
CSB-MP464487BXU
CSB-EP464487BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Gamma-glutamyl phosphate reductase (proA) CSB-YP464488BXU
CSB-EP464488BXU
CSB-BP464488BXU
CSB-MP464488BXU
CSB-EP464488BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans UPF0289 protein Bphyt_3456 (Bphyt_3456) CSB-YP464489BXU
CSB-EP464489BXU
CSB-BP464489BXU
CSB-MP464489BXU
CSB-EP464489BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Protein MraZ (mraZ) CSB-YP464490BXU
CSB-EP464490BXU
CSB-BP464490BXU
CSB-MP464490BXU
CSB-EP464490BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP464491BXU
CSB-EP464491BXU
CSB-BP464491BXU
CSB-MP464491BXU
CSB-EP464491BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Chorismate synthase (aroC) CSB-YP464492BXU
CSB-EP464492BXU
CSB-BP464492BXU
CSB-MP464492BXU
CSB-EP464492BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans UvrABC system protein C (uvrC), partial CSB-YP464493BXU
CSB-EP464493BXU
CSB-BP464493BXU
CSB-MP464493BXU
CSB-EP464493BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Probable cytosol aminopeptidase (pepA), partial CSB-YP464494BXU
CSB-EP464494BXU
CSB-BP464494BXU
CSB-MP464494BXU
CSB-EP464494BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Serine--tRNA ligase (serS) CSB-YP464495BXU
CSB-EP464495BXU
CSB-BP464495BXU
CSB-MP464495BXU
CSB-EP464495BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans ATP-dependent protease subunit HslV (hslV) CSB-YP464496BXU
CSB-EP464496BXU
CSB-BP464496BXU
CSB-MP464496BXU
CSB-EP464496BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP464497BXU
CSB-EP464497BXU
CSB-BP464497BXU
CSB-MP464497BXU
CSB-EP464497BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Potassium-transporting ATPase C chain (kdpC), partial CSB-YP464498BXU
CSB-EP464498BXU
CSB-BP464498BXU
CSB-MP464498BXU
CSB-EP464498BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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