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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Saccharomyces cerevisiae High osmolarity signaling protein SHO1 (SHO1), partial CSB-YP465059SVP1
CSB-EP465059SVP1
CSB-BP465059SVP1
CSB-MP465059SVP1
CSB-EP465059SVP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae 37S ribosomal protein RSM18, mitochondrial (RSM18), partial CSB-YP465060SVP
CSB-EP465060SVP
CSB-BP465060SVP
CSB-MP465060SVP
CSB-EP465060SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Regulator of free ubiquitin chains 1 (RFU1) CSB-YP465061SVP
CSB-EP465061SVP
CSB-BP465061SVP
CSB-MP465061SVP
CSB-EP465061SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Signal peptidase complex catalytic subunit SEC11 (SEC11), partial CSB-YP465062SVP
CSB-EP465062SVP
CSB-BP465062SVP
CSB-MP465062SVP
CSB-EP465062SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae KRR1 small subunit processome component (KRR1) CSB-YP465063SVP
CSB-EP465063SVP
CSB-BP465063SVP
CSB-MP465063SVP
CSB-EP465063SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae Protein hedgehog (hh) CSB-YP465064DKY
CSB-EP465064DKY
CSB-BP465064DKY
CSB-MP465064DKY
CSB-EP465064DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae Eukaryotic translation initiation factor 3 subunit D-2 (eIF3-S7-2), partial CSB-YP465065DKY
CSB-EP465065DKY
CSB-BP465065DKY
CSB-MP465065DKY
CSB-EP465065DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae Protein alan shepard (shep), partial CSB-YP465066DKY
CSB-EP465066DKY
CSB-BP465066DKY
CSB-MP465066DKY
CSB-EP465066DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae SAGA-associated factor 11 homolog (Sgf11) CSB-YP465067DKY
CSB-EP465067DKY
CSB-BP465067DKY
CSB-MP465067DKY
CSB-EP465067DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae Protein king tubby (king-tubby) CSB-YP465068DKY
CSB-EP465068DKY
CSB-BP465068DKY
CSB-MP465068DKY
CSB-EP465068DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae Ell-associated factor Eaf (Eaf), partial CSB-YP465069DKY
CSB-EP465069DKY
CSB-BP465069DKY
CSB-MP465069DKY
CSB-EP465069DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae Elongation factor G, mitochondrial (ico), partial CSB-YP465070DKY
CSB-EP465070DKY
CSB-BP465070DKY
CSB-MP465070DKY
CSB-EP465070DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae UPF0518 protein GF15501 (GF15501), partial CSB-YP465071DKY
CSB-EP465071DKY
CSB-BP465071DKY
CSB-MP465071DKY
CSB-EP465071DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae Protein hook (hk), partial CSB-YP465072DKY
CSB-EP465072DKY
CSB-BP465072DKY
CSB-MP465072DKY
CSB-EP465072DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae Transmembrane emp24 domain-containing protein eca (eca), partial CSB-YP465073DKY
CSB-EP465073DKY
CSB-BP465073DKY
CSB-MP465073DKY
CSB-EP465073DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae Probable cytosolic Fe-S cluster assembly factor GF22738 (GF22738) CSB-YP465074DKY
CSB-EP465074DKY
CSB-BP465074DKY
CSB-MP465074DKY
CSB-EP465074DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Integrator complex subunit 3 homolog (IntS3), partial CSB-YP465075DLK
CSB-EP465075DLK
CSB-BP465075DLK
CSB-MP465075DLK
CSB-EP465075DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Protein Smaug (smg), partial CSB-YP465076DLK
CSB-EP465076DLK
CSB-BP465076DLK
CSB-MP465076DLK
CSB-EP465076DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Serine/threonine-protein kinase GG21441 (GG21441), partial CSB-YP465077DLK
CSB-EP465077DLK
CSB-BP465077DLK
CSB-MP465077DLK
CSB-EP465077DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Ribosome biogenesis protein BOP1 homolog (GG21824), partial CSB-YP465078DLK
CSB-EP465078DLK
CSB-BP465078DLK
CSB-MP465078DLK
CSB-EP465078DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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