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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus cereus Methionine--tRNA ligase (metG), partial CSB-YP467760BQM
CSB-EP467760BQM
CSB-BP467760BQM
CSB-MP467760BQM
CSB-EP467760BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Tryptophan synthase alpha chain (trpA) CSB-YP467761BQM
CSB-EP467761BQM
CSB-BP467761BQM
CSB-MP467761BQM
CSB-EP467761BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus SsrA-binding protein (smpB) CSB-YP467762BQM
CSB-EP467762BQM
CSB-BP467762BQM
CSB-MP467762BQM
CSB-EP467762BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus HPr kinase/phosphorylase (hprK) CSB-YP467763BQM
CSB-EP467763BQM
CSB-BP467763BQM
CSB-MP467763BQM
CSB-EP467763BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP467764BQM
CSB-EP467764BQM
CSB-BP467764BQM
CSB-MP467764BQM
CSB-EP467764BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 2-isopropylmalate synthase (leuA), partial CSB-YP467765BQM
CSB-EP467765BQM
CSB-BP467765BQM
CSB-MP467765BQM
CSB-EP467765BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP467766BQM
CSB-EP467766BQM
CSB-BP467766BQM
CSB-MP467766BQM
CSB-EP467766BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Transcriptional regulator lytR (lytR), partial CSB-YP467767BQM
CSB-EP467767BQM
CSB-BP467767BQM
CSB-MP467767BQM
CSB-EP467767BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP467768BQM
CSB-EP467768BQM
CSB-BP467768BQM
CSB-MP467768BQM
CSB-EP467768BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Isopentenyl-diphosphate delta-isomerase (fni) CSB-YP467769BQM
CSB-EP467769BQM
CSB-BP467769BQM
CSB-MP467769BQM
CSB-EP467769BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Demethylmenaquinone methyltransferase (ubiE) CSB-YP467770BQM
CSB-EP467770BQM
CSB-BP467770BQM
CSB-MP467770BQM
CSB-EP467770BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Holliday junction resolvase recU (recU) CSB-YP467771BQM
CSB-EP467771BQM
CSB-BP467771BQM
CSB-MP467771BQM
CSB-EP467771BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Xanthine phosphoribosyltransferase (xpt) CSB-YP467772BQM
CSB-EP467772BQM
CSB-BP467772BQM
CSB-MP467772BQM
CSB-EP467772BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Hut operon positive regulatory protein (hutP) CSB-YP467773BQM
CSB-EP467773BQM
CSB-BP467773BQM
CSB-MP467773BQM
CSB-EP467773BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Antiholin-like protein LrgA (lrgA), partial CSB-YP467774BQM1
CSB-EP467774BQM1
CSB-BP467774BQM1
CSB-MP467774BQM1
CSB-EP467774BQM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S18 (rpsR) CSB-YP467775BQM
CSB-EP467775BQM
CSB-BP467775BQM
CSB-MP467775BQM
CSB-EP467775BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus DNA mismatch repair protein mutL (mutL), partial CSB-YP467776BQM
CSB-EP467776BQM
CSB-BP467776BQM
CSB-MP467776BQM
CSB-EP467776BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Ribosome maturation factor RimP (rimP) CSB-YP467777BQM
CSB-EP467777BQM
CSB-BP467777BQM
CSB-MP467777BQM
CSB-EP467777BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus GTP-sensing transcriptional pleiotropic repressor CodY (codY) CSB-YP467778BQM
CSB-EP467778BQM
CSB-BP467778BQM
CSB-MP467778BQM
CSB-EP467778BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Ribonuclease HII (rnhB) CSB-YP467779BQM
CSB-EP467779BQM
CSB-BP467779BQM
CSB-MP467779BQM
CSB-EP467779BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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