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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus cereus ATP-dependent helicase/nuclease subunit A (addA), partial CSB-YP470100BQM
CSB-EP470100BQM
CSB-BP470100BQM
CSB-MP470100BQM
CSB-EP470100BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0753 protein BCAH820_3189 (BCAH820_3189), partial CSB-YP470101BQM
CSB-EP470101BQM
CSB-BP470101BQM
CSB-MP470101BQM
CSB-EP470101BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus N- (5'-phosphoribosyl)anthranilate isomerase CSB-YP470102BQM
CSB-EP470102BQM
CSB-BP470102BQM
CSB-MP470102BQM
CSB-EP470102BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Lactate utilization protein C (lutC) CSB-YP470103BQM
CSB-EP470103BQM
CSB-BP470103BQM
CSB-MP470103BQM
CSB-EP470103BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Phosphoglycerate kinase (pgk) CSB-YP470104BQM
CSB-EP470104BQM
CSB-BP470104BQM
CSB-MP470104BQM
CSB-EP470104BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 7-cyano-7-deazaguanine synthase (queC) CSB-YP470105BQM
CSB-EP470105BQM
CSB-BP470105BQM
CSB-MP470105BQM
CSB-EP470105BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP470106BQM
CSB-EP470106BQM
CSB-BP470106BQM
CSB-MP470106BQM
CSB-EP470106BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Phosphoribosyl-AMP cyclohydrolase (hisI) CSB-YP470107BQM
CSB-EP470107BQM
CSB-BP470107BQM
CSB-MP470107BQM
CSB-EP470107BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Phosphomethylpyrimidine synthase (thiC), partial CSB-YP470108BQM
CSB-EP470108BQM
CSB-BP470108BQM
CSB-MP470108BQM
CSB-EP470108BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus ATP synthase gamma chain (atpG) CSB-YP470109BQM
CSB-EP470109BQM
CSB-BP470109BQM
CSB-MP470109BQM
CSB-EP470109BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Cytidylate kinase (cmk) CSB-YP470110BQM
CSB-EP470110BQM
CSB-BP470110BQM
CSB-MP470110BQM
CSB-EP470110BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Methylglyoxal synthase (mgsA) CSB-YP470111BQM
CSB-EP470111BQM
CSB-BP470111BQM
CSB-MP470111BQM
CSB-EP470111BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0061 protein BCAH820_3523 (BCAH820_3523) CSB-YP470112BQM
CSB-EP470112BQM
CSB-BP470112BQM
CSB-MP470112BQM
CSB-EP470112BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0741 protein BCAH820_5476 (BCAH820_5476) CSB-YP470113BQM
CSB-EP470113BQM
CSB-BP470113BQM
CSB-MP470113BQM
CSB-EP470113BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Cell cycle protein GpsB (gpsB) CSB-YP470114BQM
CSB-EP470114BQM
CSB-BP470114BQM
CSB-MP470114BQM
CSB-EP470114BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Small, acid-soluble spore protein O (sspO) CSB-YP470115BQM
CSB-EP470115BQM
CSB-BP470115BQM
CSB-MP470115BQM
CSB-EP470115BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0154 protein BCAH820_3697 (BCAH820_3697), partial CSB-YP470116BQM
CSB-EP470116BQM
CSB-BP470116BQM
CSB-MP470116BQM
CSB-EP470116BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L9 (rplI) CSB-YP470117BQM
CSB-EP470117BQM
CSB-BP470117BQM
CSB-MP470117BQM
CSB-EP470117BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Ribonuclease P protein component (rnpA) CSB-YP470118BQM
CSB-EP470118BQM
CSB-BP470118BQM
CSB-MP470118BQM
CSB-EP470118BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Peptidase T (pepT) CSB-YP470119BQM
CSB-EP470119BQM
CSB-BP470119BQM
CSB-MP470119BQM
CSB-EP470119BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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