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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Bacillus cereus NH (3)-dependent NAD (+) synthetase CSB-YP476303BQL
CSB-EP476303BQL
CSB-BP476303BQL
CSB-MP476303BQL
CSB-EP476303BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP476304BQL
CSB-EP476304BQL
CSB-BP476304BQL
CSB-MP476304BQL
CSB-EP476304BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Formate--tetrahydrofolate ligase (fhs), partial CSB-YP476305BQL
CSB-EP476305BQL
CSB-BP476305BQL
CSB-MP476305BQL
CSB-EP476305BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Putative phosphotransferase BCAH187_A4429 (BCAH187_A4429) CSB-YP476306BQL
CSB-EP476306BQL
CSB-BP476306BQL
CSB-MP476306BQL
CSB-EP476306BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Ribosomal protein L11 methyltransferase (prmA) CSB-YP476307BQL
CSB-EP476307BQL
CSB-BP476307BQL
CSB-MP476307BQL
CSB-EP476307BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP476308BQL
CSB-EP476308BQL
CSB-BP476308BQL
CSB-MP476308BQL
CSB-EP476308BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Glutamine amidotransferase subunit pdxT (pdxT) CSB-YP476309BQL
CSB-EP476309BQL
CSB-BP476309BQL
CSB-MP476309BQL
CSB-EP476309BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Bifunctional protein GlmU (glmU) CSB-YP476310BQL
CSB-EP476310BQL
CSB-BP476310BQL
CSB-MP476310BQL
CSB-EP476310BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0346 protein BCAH187_A2410 (BCAH187_A2410) CSB-YP476311BQL
CSB-EP476311BQL
CSB-BP476311BQL
CSB-MP476311BQL
CSB-EP476311BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Aspartate--tRNA ligase (aspS), partial CSB-YP476312BQL
CSB-EP476312BQL
CSB-BP476312BQL
CSB-MP476312BQL
CSB-EP476312BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP476313BQL
CSB-EP476313BQL
CSB-BP476313BQL
CSB-MP476313BQL
CSB-EP476313BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Probable GTP-binding protein EngB (engB) CSB-YP476314BQL
CSB-EP476314BQL
CSB-BP476314BQL
CSB-MP476314BQL
CSB-EP476314BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Ribonuclease PH (rph) CSB-YP476315BQL
CSB-EP476315BQL
CSB-BP476315BQL
CSB-MP476315BQL
CSB-EP476315BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Cysteine--tRNA ligase (cysS) CSB-YP476316BQL
CSB-EP476316BQL
CSB-BP476316BQL
CSB-MP476316BQL
CSB-EP476316BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP476317BQL
CSB-EP476317BQL
CSB-BP476317BQL
CSB-MP476317BQL
CSB-EP476317BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S10 (rpsJ) CSB-YP476318BQL
CSB-EP476318BQL
CSB-BP476318BQL
CSB-MP476318BQL
CSB-EP476318BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L22 (rplV) CSB-YP476319BQL
CSB-EP476319BQL
CSB-BP476319BQL
CSB-MP476319BQL
CSB-EP476319BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L5 (rplE) CSB-YP476320BQL
CSB-EP476320BQL
CSB-BP476320BQL
CSB-MP476320BQL
CSB-EP476320BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L15 (rplO) CSB-YP476321BQL
CSB-EP476321BQL
CSB-BP476321BQL
CSB-MP476321BQL
CSB-EP476321BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L36 (rpmJ) CSB-YP476322BQL
CSB-EP476322BQL
CSB-BP476322BQL
CSB-MP476322BQL
CSB-EP476322BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據他們開發(fā)重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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