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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Thermococcus onnurineus 30S ribosomal protein S7P (rps7p) CSB-YP478423TMR
CSB-EP478423TMR
CSB-BP478423TMR
CSB-MP478423TMR
CSB-EP478423TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Probable hydrogenase nickel incorporation protein HypA (hypA) CSB-YP478424TMR
CSB-EP478424TMR
CSB-BP478424TMR
CSB-MP478424TMR
CSB-EP478424TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Probable L-threonine 3-dehydrogenase (tdh) CSB-YP478425TMR
CSB-EP478425TMR
CSB-BP478425TMR
CSB-MP478425TMR
CSB-EP478425TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus DNA-directed RNA polymerase subunit P (rpoP) CSB-YP478426TMR
CSB-EP478426TMR
CSB-BP478426TMR
CSB-MP478426TMR
CSB-EP478426TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Enolase (eno) CSB-YP478427TMR
CSB-EP478427TMR
CSB-BP478427TMR
CSB-MP478427TMR
CSB-EP478427TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Tryptophan--tRNA ligase (trpS) CSB-YP478428TMR
CSB-EP478428TMR
CSB-BP478428TMR
CSB-MP478428TMR
CSB-EP478428TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus UPF0127 protein TON_0581 (TON_0581) CSB-YP478429TMR
CSB-EP478429TMR
CSB-BP478429TMR
CSB-MP478429TMR
CSB-EP478429TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Putative snRNP Sm-like protein (TON_0644) CSB-YP478430TMR
CSB-EP478430TMR
CSB-BP478430TMR
CSB-MP478430TMR
CSB-EP478430TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus V-type ATP synthase subunit C (atpC) CSB-YP478431TMR
CSB-EP478431TMR
CSB-BP478431TMR
CSB-MP478431TMR
CSB-EP478431TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Ribonuclease P protein component 2 (rnp2) CSB-YP478432TMR
CSB-EP478432TMR
CSB-BP478432TMR
CSB-MP478432TMR
CSB-EP478432TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Cyclic 2,3-diphosphoglycerate synthetase (cpgS) CSB-YP478433TMR
CSB-EP478433TMR
CSB-BP478433TMR
CSB-MP478433TMR
CSB-EP478433TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Pyridoxal biosynthesis lyase pdxS (pdxS) CSB-YP478434TMR
CSB-EP478434TMR
CSB-BP478434TMR
CSB-MP478434TMR
CSB-EP478434TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus tRNA (pseudouridine (54)-N (1))-methyltransferase CSB-YP478435TMR
CSB-EP478435TMR
CSB-BP478435TMR
CSB-MP478435TMR
CSB-EP478435TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Translation initiation factor 2 subunit gamma (eIF2G) CSB-YP478436TMR
CSB-EP478436TMR
CSB-BP478436TMR
CSB-MP478436TMR
CSB-EP478436TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP478437TMR
CSB-EP478437TMR
CSB-BP478437TMR
CSB-MP478437TMR
CSB-EP478437TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Glyoxylate reductase (gyaR) CSB-YP478438TMR
CSB-EP478438TMR
CSB-BP478438TMR
CSB-MP478438TMR
CSB-EP478438TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Probable tyrosine recombinase XerC-like (TON_1013) CSB-YP478439TMR
CSB-EP478439TMR
CSB-BP478439TMR
CSB-MP478439TMR
CSB-EP478439TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus DNA-binding protein TON_1102 (TON_1102) CSB-YP478440TMR
CSB-EP478440TMR
CSB-BP478440TMR
CSB-MP478440TMR
CSB-EP478440TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus 3-dehydroquinate dehydratase (aroD) CSB-YP478441TMR
CSB-EP478441TMR
CSB-BP478441TMR
CSB-MP478441TMR
CSB-EP478441TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Probable RNA 2'-phosphotransferase (kptA) CSB-YP478442TMR
CSB-EP478442TMR
CSB-BP478442TMR
CSB-MP478442TMR
CSB-EP478442TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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