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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus cereus 30S ribosomal protein S8 (rpsH) CSB-YP481944BQL
CSB-EP481944BQL
CSB-BP481944BQL
CSB-MP481944BQL
CSB-EP481944BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S13 (rpsM) CSB-YP481945BQL
CSB-EP481945BQL
CSB-BP481945BQL
CSB-MP481945BQL
CSB-EP481945BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Anhydro-N-acetylmuramic acid kinase (anmK) CSB-YP481946BQL
CSB-EP481946BQL
CSB-BP481946BQL
CSB-MP481946BQL
CSB-EP481946BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP481947BQL
CSB-EP481947BQL
CSB-BP481947BQL
CSB-MP481947BQL
CSB-EP481947BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0756 membrane protein BCAH187_A4721 (BCAH187_A4721), partial CSB-YP481948BQL1
CSB-EP481948BQL1
CSB-BP481948BQL1
CSB-MP481948BQL1
CSB-EP481948BQL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Redox-sensing transcriptional repressor rex (rex) CSB-YP481949BQL
CSB-EP481949BQL
CSB-BP481949BQL
CSB-MP481949BQL
CSB-EP481949BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Phosphoribosylformylglycinamidine synthase 2 (purL), partial CSB-YP481950BQL
CSB-EP481950BQL
CSB-BP481950BQL
CSB-MP481950BQL
CSB-EP481950BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S4 (rpsD) CSB-YP481951BQL
CSB-EP481951BQL
CSB-BP481951BQL
CSB-MP481951BQL
CSB-EP481951BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus tRNA (guanine-N (7)-)-methyltransferase CSB-YP481952BQL
CSB-EP481952BQL
CSB-BP481952BQL
CSB-MP481952BQL
CSB-EP481952BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Heptaprenylglyceryl phosphate synthase (pcrB) CSB-YP481953BQL
CSB-EP481953BQL
CSB-BP481953BQL
CSB-MP481953BQL
CSB-EP481953BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Putative metal-dependent hydrolase BCAH187_A2766 (BCAH187_A2766) CSB-YP481954BQL
CSB-EP481954BQL
CSB-BP481954BQL
CSB-MP481954BQL
CSB-EP481954BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0374 protein BCAH187_A0583 (BCAH187_A0583) CSB-YP481955BQL
CSB-EP481955BQL
CSB-BP481955BQL
CSB-MP481955BQL
CSB-EP481955BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Pyrrolidone-carboxylate peptidase (pcp) CSB-YP481956BQL
CSB-EP481956BQL
CSB-BP481956BQL
CSB-MP481956BQL
CSB-EP481956BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Potassium-transporting ATPase B chain (kdpB), partial CSB-YP481957BQL1
CSB-EP481957BQL1
CSB-BP481957BQL1
CSB-MP481957BQL1
CSB-EP481957BQL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus HPr kinase/phosphorylase (hprK) CSB-YP481958BQL
CSB-EP481958BQL
CSB-BP481958BQL
CSB-MP481958BQL
CSB-EP481958BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP481959BQL
CSB-EP481959BQL
CSB-BP481959BQL
CSB-MP481959BQL
CSB-EP481959BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus ATP synthase subunit c (atpE), partial CSB-YP481960BQL1
CSB-EP481960BQL1
CSB-BP481960BQL1
CSB-MP481960BQL1
CSB-EP481960BQL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L31 type B (rpmE2) CSB-YP481961BQL
CSB-EP481961BQL
CSB-BP481961BQL
CSB-MP481961BQL
CSB-EP481961BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Homoserine O-succinyltransferase (metA) CSB-YP481962BQL
CSB-EP481962BQL
CSB-BP481962BQL
CSB-MP481962BQL
CSB-EP481962BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0061 protein BCAH187_A3529 (BCAH187_A3529) CSB-YP481963BQL
CSB-EP481963BQL
CSB-BP481963BQL
CSB-MP481963BQL
CSB-EP481963BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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