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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Acidithiobacillus ferrooxidans GTPase obg (obg) CSB-YP482124AWH
CSB-EP482124AWH
CSB-BP482124AWH
CSB-MP482124AWH
CSB-EP482124AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Transcriptional repressor NrdR (nrdR) CSB-YP482125AWH
CSB-EP482125AWH
CSB-BP482125AWH
CSB-MP482125AWH
CSB-EP482125AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 50S ribosomal protein L7/L12 (rplL) CSB-YP482126AWH
CSB-EP482126AWH
CSB-BP482126AWH
CSB-MP482126AWH
CSB-EP482126AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 50S ribosomal protein L3 (rplC) CSB-YP482127AWH
CSB-EP482127AWH
CSB-BP482127AWH
CSB-MP482127AWH
CSB-EP482127AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 50S ribosomal protein L29 (rpmC) CSB-YP482128AWH
CSB-EP482128AWH
CSB-BP482128AWH
CSB-MP482128AWH
CSB-EP482128AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 30S ribosomal protein S8 (rpsH) CSB-YP482129AWH
CSB-EP482129AWH
CSB-BP482129AWH
CSB-MP482129AWH
CSB-EP482129AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 50S ribosomal protein L15 (rplO) CSB-YP482130AWH
CSB-EP482130AWH
CSB-BP482130AWH
CSB-MP482130AWH
CSB-EP482130AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP482131AWH
CSB-EP482131AWH
CSB-BP482131AWH
CSB-MP482131AWH
CSB-EP482131AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP482132AWH
CSB-EP482132AWH
CSB-BP482132AWH
CSB-MP482132AWH
CSB-EP482132AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Proline--tRNA ligase (proS), partial CSB-YP482133AWH
CSB-EP482133AWH
CSB-BP482133AWH
CSB-MP482133AWH
CSB-EP482133AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans S-adenosylmethionine synthase (metK) CSB-YP482134AWH
CSB-EP482134AWH
CSB-BP482134AWH
CSB-MP482134AWH
CSB-EP482134AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Na (+)/H (+) antiporter nhaA, partial CSB-YP482135AWH
CSB-EP482135AWH
CSB-BP482135AWH
CSB-MP482135AWH
CSB-EP482135AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Thiazole synthase (thiG) CSB-YP482136AWH
CSB-EP482136AWH
CSB-BP482136AWH
CSB-MP482136AWH
CSB-EP482136AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Ketol-acid reductoisomerase (ilvC) CSB-YP482137AWH
CSB-EP482137AWH
CSB-BP482137AWH
CSB-MP482137AWH
CSB-EP482137AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP482138AWH
CSB-EP482138AWH
CSB-BP482138AWH
CSB-MP482138AWH
CSB-EP482138AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Phosphomethylpyrimidine synthase (thiC), partial CSB-YP482139AWH
CSB-EP482139AWH
CSB-BP482139AWH
CSB-MP482139AWH
CSB-EP482139AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Threonine--tRNA ligase (thrS), partial CSB-YP482140AWH
CSB-EP482140AWH
CSB-BP482140AWH
CSB-MP482140AWH
CSB-EP482140AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Chaperone protein DnaJ (dnaJ) CSB-YP482141AWH
CSB-EP482141AWH
CSB-BP482141AWH
CSB-MP482141AWH
CSB-EP482141AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Methionine--tRNA ligase (metG), partial CSB-YP482142AWH
CSB-EP482142AWH
CSB-BP482142AWH
CSB-MP482142AWH
CSB-EP482142AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 50S ribosomal protein L9 (rplI) CSB-YP482143AWH
CSB-EP482143AWH
CSB-BP482143AWH
CSB-MP482143AWH
CSB-EP482143AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據他們開發(fā)重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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